血清N-糖组在原发性肺癌中的特征性表达及与中医证型的关联性

Characteristic expression of serum N-glycomic in primary lung cancer and its correlation with traditional Chinese medicine syndromes

目的:分析原发性肺癌患者外周血N-糖组图谱的特征性表达,探索外周血N-糖组学对原发性肺癌患者中医证型的关联性。方法:分别收集原发性肺癌患者、良性肺部疾病患者、健康对照者外周血,基于DNA测序仪的荧光糖电泳(DSA-FACE)技术对血清N-糖组图谱进行分析。进一步探索原发性肺癌患者外周血N-糖组与肺癌分期、中医证型之间的相关性。结果:外周血N-糖组图谱检测结果显示,Peak2、Peak3、Peak4、Peak5、Peak6、Peak9、Peak10、Peak11、Peak12及总核心岩藻糖基化水平(SumFuc)在原发性肺癌患者、良性肺部疾病患者及健康对照者中存在差异性(P<0.05);从健康对照组到良性肺部疾病再到原发性肺癌组,Peak2、Peak5、Peak9呈逐步上升趋势;而Peak3、Peak4、Peak6、SumFuc呈逐步下降趋势。原发性肺癌组N-糖组Peak3、Peak4、Peak6、SumFuc与TNM分期呈负相关,Peak9、Peak12与TNM分期呈正相关。N-糖组在不同证型患者的差异具有统计学意义(P<0.05)。Peak1、Peak6、SumFuc从气虚痰湿证、阴虚热毒证到气阴两虚证中呈逐步下降趋势。Peak5、Peak11在3种证型中呈逐步上升趋势。结论:原发性肺癌患者外周血N-糖组存在特征性表达,N-糖组在原发性肺癌筛查和鉴别方面具有潜在的应用价值。外周血N-糖组与原发性肺癌患者TNM分期以及中医证型分布具有相关性。

Objective:To analyze the characteristic expression of serum N-glycome profiles of patients with primary lung cancer and explore it’s correlation with TCM syndromes.Methods:The peripheral blood of patients with primary lung cancer,patients with benign lung diseases and healthy controls were collected,respectively;and the serum N-glycomics were analyzed by DNA sequencer-assisted,fluorophore-assisted carbohydrate electrophoresis(DSA-FACE).Furthermore,the correlation between N-glycom profiles,TNM stage and TCM syndrome was explored.Results:The N-glycom profiles showed Peak2,Peak3,Peak4,Peak5,Peak6,Peak9,Peak10,Peak11,Peak12 and SumFuc were different in primary lung cancer,benign lung diseases and healthy controls(P<0.05).From the healthy control to the benign lung diseases and to the primary lung cancer patients,Peak2,Peak5,Peak9 showed a gradual upward trend,while Peak3,Peak4,Peak6 and SumFuc showed a gradual downward trend.Peak3,Peak4,Peak6 and SumFuc were negatively correlated with lung cancer staging,while Peak9,Peak12 were positively correlated with TNM staging.The N-glycome profiles and diagnostic models had statistically significant differences in patients with different syndrome(P<0.05).Peak1,Peak6 and SumFuc showed a gradual downward trend from qi deficiency and phlegm-dampness syndrome,deficiency yin and excessive heat syndrome to qi and yin deficiency syndrome.Peak5 and Peak11 value showed a gradual upward trend among the those syndromes.Conclusion:The serum N-glycome profiles of the primary lung cancer patients changed distinguishably.The N-glycome profiles have potential application value in screening and diagnosis of primary lung cancer.The N-glycome profiles of peripheral blood are correlated with TNM staging and TCM syndromes in patients with primary lung cancer.