康达心口服液对心肾综合征大鼠氧化应激的影响

Effect of Kangdaxin Oral Liquid on oxidative stress in rats with cardiorenal syndrome

目的探讨康达心口服液对心肾综合征(CRS)大鼠的影响及潜在机制。方法将60只SD大鼠随机分为5组:假手术(sham)组、心力衰竭(HF)组、CRS组、HF干预(HF+I)组、CRS干预(CRS+I)组,通过心脏彩超测量各组大鼠左室射血分数(LVEF)、左室缩短分数(FS);通过ELISA法检测血清氨基末端脑钠肽前体(NT-proBNP)、肌酐(Cr)、尿素氮(Bun)、总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平。结果HF组、HF+I组、CRS组、CRS+I组大鼠的FS及LVEF值低于sham组,血清NT-proBNP水平高于sham组,差异有统计学意义(P<0.05)。HF+I组的FS及LVEF值高于HF组,NT-proBNP水平低于HF组,差异有统计学意义(P<0.05)。CRS+I组的FS及LVEF值高于CRS组,NT-proBNP水平低于CRS组,差异有统计学意义(P<0.05)。CRS组、CRS+I组大鼠的血清Cr及Bun水平高于sham组,差异有统计学意义(P<0.05)。CRS+I组的血清Cr及Bun水平低于CRS组,差异有统计学意义(P<0.05)。HF组、HF+I组、CRS组、CRS+I组的血清T-AOC、SOD水平低于sham组,MDA水平高于sham组,差异有统计学意义(P<0.05)。HF+I组大鼠的血清T-AOC、SOD水平高于HF组、MDA水平低于HF组,差异有统计学意义(P<0.05)。CRS+I组大鼠的血清TAOC、SOD水平高于CRS组,MDA水平低于CRS组,差异有统计学意义(P<0.05)。结论康达心改善CRS大鼠心功能的效应,可能是通过提高抗氧化能力、抑制氧化应激实现的。

Objective To investigate the effect and potential mechanism of Kangdaxin Oral Liquid on cardiorenal syndrome(CRS)rats.Methods Sixty SD rats were randomly divided into 5 groups:sham operation(sham)group,heart failure(HF)group,CRS group,HF+intervention(I)group and CRS+I group.Left ventricular ejection fraction(LVEF)and left ventricular shortening fraction(FS)were measured by cardiac color ultrasound.The levels of serum N-terminal pro-B type natriuretic peptide(NT-proBNP),creatinine(Cr),urea nitrogen(Bun),total antioxidant capacity(T-AOC),superoxide dismutase(SOD),malondialdehyde(MDA)were determined by ELISA.Results The FS and LVEF values of rats in HF group,HF+I group,CRS group and CRS+I group were lower than those in sham group,and serum NT-proBNP level was higher than that in sham group,the differences were statistically significant(P<0.05).The FS and LVEF values in HF+I group were higher than those in HF group,and the level of NT-proBNP was lower than that in HF group,the differences were statistically significant(P<0.05).The FS and LVEF values of CRS+I group were higher than those of CRS group,and the level of NT-proBNP was lower than that of CRS group,the differences were statistically significant(P<0.05).The levels of serum Cr and Bun in CRS group and CRS+I group were higher than those in sham group,and the differences were statistically significant(P<0.05).The levels of serum Cr and Bun in CRS+I group were lower than those in CRS group,the differences were statistically significant(P<0.05).Serum T-AOC and SOD levels in HF group,HF+I group,CRS group and CRS+I group were lower than those in sham group,while MDA level was higher than that in sham group,the differences were statistically significant(P<0.05).Serum T-AOC and SOD levels in HF+I group were higher than those in HF group,while MDA levels were lower than those in HF group,the differences were statistically significant(P<0.05).Serum T-AOC and SOD levels in CRS+I group were higher than those in CRS group,while MDA levels were lower than those in CRS group,the differences were statistically significant(P<0.05).Conclusion Kangdaxin Oral Liquid may improve cardiac function in rats with cardiorenal syndrome by promoting antioxidant capacity and suppressing oxidative stress.