宏基因组测序在骨关节感染诊断中的应用前景

The application prospects of metagenomic next-generation sequencing in the diagnosis of osteoarticular infections

骨关节感染(osteoarticular infections, OAIs)在临床上仍然是一种危害严重的疾病。准确和早期发现引起骨关节感染的病原体,对于实施临床治疗很重要。传统的技术检测速度慢、阳性率低。因此,特别需要发展出更好的方法或技术来确定引起骨关节感染的病原体。近年来,越来越多的研究报道了宏基因组测序(metagenomic next-generation sequencing, mNGS)检测骨关节感染病原体速度快、特异性和灵敏度高,可作为有效的诊断工具。本文总结了主要传统技术在骨关节感染病原体诊断中的应用,并讨论了宏基因组测序在骨关节感染病原体诊断中的应用前景。

Osteoarticular infections(OAIs) are still serious diseases in clinical practice. Accurate and early detection of the pathogens that cause osteoarticular infections remains important for administering clinical treatment, however, the traditional detection methods is time consuming with lower positive rate. Therefore, it is necessary to develop better methods or techniques for determining the pathogens responsible for OAIs. In recent years, an increasing number of studies have reported that metagenomic next-generation sequencing(mNGS) is a fast approach with high specificity and sensitivity, and has potential to be used as an effective diagnostic tool. This article summarizes the application of major traditional techniques in the diagnosis of OAIs, and discuss the prospects of mNGS in the diagnosis of OAIs.