酶交联螺蛳蛋白/Ca^(2+)絮凝剂处理高浊度废水的研究

Enzyme-crosslinked mud snail protein/Ca^(2+) flocculant for treatment of high turbidity wastewater

以螺蛳蛋白(Msp)为原料,通过转谷氨酰胺酶(TGase)钙双交联反应制备了大分子质量的酶交联螺蛳蛋白/Ca^(2+)絮凝剂(TGase-Msp/Ca^(2+)),利用FT-IR、SEM-EDS、Zeta电位对其结构进行表征,考察了TGase-Msp/Ca^(2+)对高岭土悬浮液絮凝性能的影响。结果表明,Ca^(2+)浓度影响TGase-Msp/Ca^(2+)的絮凝性能,在Ca^(2+)浓度为0.2 mol/L时TGase-Msp/Ca^(2+)因具有良好的桥接作用和电荷中和能力,表现出优秀的絮凝性能并具有较小污泥体积。在TGase-Msp/Ca^(2+)用量为1.25 mL、pH为6时,浊度去除率达到99.89%,污泥体积分数为28 mL/L。同时,TGase-Msp/Ca^(2+)具有很宽的絮凝范围,在pH 2~12的范围内去除率达到97%以上,絮体粒径达到18μm,沉降速度为2.21 mm/s。

A large molecular weight enzyme-crosslinked mud snail protein/Ca^(2+)flocculant(TGase-Msp/Ca^(2+))is prepared through double cross-linking with transglutaminase(TGase)and calcium with mud snail protein(Msp)as raw materials,and its structure is characterized by FT-IR,Zeta,SEM-EDS.The influences of TGase-Msp/Ca^(2+)on the flocculation performance of kaolin suspensions are investigated.It is verified that Ca^(2+)content influences the flocculation performance of TGase-Msp/Ca^(2+).As Ca^(2+)content is 0.2 mol·L^(-1),TGase-Msp/Ca^(2+)has good bridging and charge neutralization ability,which makes it exhibit excellent flocculation performance and generate a small sludge volume.The removal rate of turbidity reaches 99.89%and the volume fraction of sludge is 28 mL·L^(-1) when pH is 6 and the dosage of TGase-Msp/Ca^(2+)is 1.25 mL.In addition,TGase-Msp/Ca^(2+)has a wide flocculation range.The removal rate of turbidity can exceed 97%in the range of pH 2-12.Meanwhile,the floc particle size of TGase-Msp/Ca^(2+)reaches 18μm with a settling rate of 2.21 mm·L^(-1).