α-氯醛糖与右美托咪定对大鼠直肠给药麻醉效果的综合评价

Comprehensive evaluation of the effect ofα-chloraldose and dexmedetomidine on rectal anesthesia in rats

为了探讨α-氯醛糖和右美托咪定联合应用对大鼠直肠给药麻醉的效果,使用13 mg/kg的α-氯醛糖、0.6 mg/kg的右美托咪定对10只健康SD大鼠实施直肠给药麻醉,并对麻醉过程中大鼠的麻醉时间、作用效果、基本生理指标及血常规和血液生化指标进行监测,对2种麻醉药物复合使用进行综合评价。结果显示:麻醉诱导时间为(9.50±2.30)min、维持时间为(73.70±7.56)min、苏醒时间为(28.10±3.12)min,呈现出诱导和苏醒快速、维持时间长的特点。镇静、镇痛及肌松效果良好,可提供约60 min良好的麻醉作用时间。常规生理指标结果显示,大鼠麻醉全程脉搏血氧饱和度平均为(96.54±1.52)%,体温、呼吸频率和心率变化趋势均表现为先降低后升高,分别在服药后60、60和40 min降至最低值,而后逐步升高,直至监测结束(服药后240 min)。实验室检测结果显示,给药后,大鼠的血常规、肝功能及肾功能指标均在参考范围内,对其无损害。综合各部分结果表明,α-氯醛糖联合右美托咪定对大鼠进行直肠麻醉具有麻醉持续时间较长、麻醉效果良好均衡等优点,对常规生理指标干扰较明显,表现为先抑制后恢复的趋势,所测血常规、肝功能及肾功能指标均在正常范围内。

In order to investigate the anesthetic effect ofα-chloralose and dexmedetomidine on rectal anesthesia in rats,10 healthy SD rats were anesthetized.The anesthetic time,effect,basic physiological indexes,blood routine and blood biochemical indexes were monitored,and the combined use of the two anesthetics was comprehensively evaluated.The results showed that the induction time,maintenance time and recovery time of anesthesia were(9.50±2.30)min,(73.70±7.56)min and(28.10±3.12)min,respectively,which showed the characteristics of rapid induction and recovery and long maintenance time.The effects of sedation,analgesia and muscle relaxation of the rats were good,which provided a good anesthetic time of about 60min.The results of regular physiological index test showed that the average pulse oxygen saturation during anesthesia was(96.54±1.52)%.The changes in their body temperature,respiratory rate and heart rate decreased first and then increased,reaching the lowest values at 60 min,40 min,and 60 min,respectively,after administration,and then gradually increased until the end of monitoring(240 min after administration).The laboratory test showed that,after administration,the blood routine,liver function and renal function indexes of the rats were all within the reference range,and there was no damage to them.The results of all the experiments indicated that the rectal anesthesia of rats withα-chloralose combined with dexmetomidine had the advantages of long anesthetic duration,good and balanced anesthetic effect,and obvious interference on routine physiological indexes,exhibiting a trend of inhibition first and then recovery,with no obvious sequela after anesthesia.