多粘类芽孢杆菌LY1的全基因组测序及分析

Whole-Genome Sequencing and Analysis on Paenibacillus polymyxa LY1

为全面了解多粘类芽孢杆菌LY1的遗传背景,利用基因组测序技术全面解析LY1菌株的基因组序列,再通过生物信息学的方法比较不同菌株基因间的差异性和相似性,获得LY1菌株的基因组基本特征、基因功能注释及分类、系统发育进化关系等关键信息,从全基因组水平了解其系统发育进化关系。结果表明:多粘类芽孢杆菌LY1 的基因组长度为5 765 474 bp,共有6 086个蛋白质编码基因、162个 tRNA 基因、42个rRNA 基因,GC含量约为45.23%;在GO功能注释中,在生物过程分类下代谢过程和细胞过程中的基因占比最多,分别占比40.77%和38.98%;在细胞组分分类下,分类到细胞和细胞组成中的基因占比最多,为35.18%;在分子功能分类下,分类到催化活性和结合中的基因占比最多,分别为40.34%和35.78%;系统发育进化分析显示,菌株LY1与Paenibacillus polymyxa M1、Paenibacillus polymyxa OSY-DF、Paenibacillus polymyxa SC2、Paenibacillus polymyxa E681等菌株进化距离较近。

In order to study the genome structure and function of Paenibacillus polymyxa LY1,whole-genome sequencing technology was used for sequencing and analysis.The results showed that the genome of Paenibacillus polymyxa (P.p) LY1 contained 5,765,474bp,6,086 protein-coding genes,162 tRNA genes,and 42 rRNA genes,and the GC content was about 45.23%.GO function annotations showed that genes in metabolic processes and cellular processes occupied the largest proportion under the biological process classification,accounting for 40.77% and 38.98% respectively;under the classification of cell components,the proportion of genes classified into cell and cell part was the largest,at 35.18%;under the molecular function classification,the genes classified into catalytic activity and binding accounted for the largest proportion,40.34% and 35.78%,respectively.Phylogenetic evolutionary analysis showed that P.p LY1 was close to P.p M1,P.p OSY-DF,P.p SC2,and P.p E681.In this study,the basic genome-wide characteristics of P.p LY1 were obtained,which provided a basis for subsequent genetic study of Paenibacillus polymyxa.