雷公藤多苷片对类风湿关节炎大鼠肠道菌群的影响

Effects of Tripterygium Glycosides Tablets on Gut Microbiota in Rats with Rheumatoid Arthritis

目的:考察胶原诱导的关节炎(CIA)大鼠给予雷公藤多苷片6周后,对关节炎症状、主要脏器和肠道菌群的影响。方法:选取雄性SD大鼠随机分为正常组和CIA模型组,其中CIA模型组采用牛Ⅱ型胶原和不完全弗氏佐剂诱导类风湿关节炎。造模成功后,CIA大鼠灌胃给予溶剂或雷公藤多苷片混悬液(90 mg·kg^(-1)),给药期间观察大鼠的一般状况并记录足趾容积。6周后,检测雷公藤多苷片的抗炎效果,以及大鼠的肝肾情况和肠道菌群变化。结果:与模型组相比,给予6周雷公藤多苷片后,CIA大鼠关节肿胀程度减轻(P<0.05),炎症因子IL⁃6水平降低(P<0.01),TNF⁃α水平降低(P<0.05);给药期间大鼠未见异常症状;肝功能及病理切片与正常对照组无明显差异,肾脏发生轻微肾小球囊腔狭窄、粘连;肠道菌群结构发生改变,其中有益菌群增加,条件致病菌群减少。结论:雷公藤多苷片长期给药对CIA大鼠的抗炎治疗作用与对肠道菌群的影响有关。

Objective:To investigate the effects of oral administration of tripterygium glycosides tablets on arthritis symptoms,ma⁃jor organs and gut microbiota in collagen⁃induced arthritis(CIA)rats after 6 weeks.Methods:Male SD rats were randomly divided in⁃to normal group and CIA model group.CIA model group was induced by bovine type II collagen and incomplete Freund’s adjuvant.Af⁃ter successful modeling,CIA model group was given solvent or tripterygium glycosides tablet suspension(90 mg·kg^(-1))by oral admin⁃istration.The general condition was observed and the change of paw volume was recorded.After six weeks,the therapeutic effect,liver and kidney conditions,and gut microbiota changes were detected.Results:After treatment with tripterygium glycosides tablets,the de⁃gree of joint swelling and the levels of inflammatory factors IL⁃6 and TNF⁃αin rheumatoid arthritis rats were reduced(P<0.05 or P<0.01).No abnormal clinical symptoms were found in rats during administration.There were no significant differences in liver function and pathological section compared with those in the control group,while slight glomerular cystic stenosis and adhesion occurred in kid⁃ney.The structure of gut microbiota was changed,in which the beneficial microbiota increased and the conditional pathogenic bacteria decreased.Conclusion:The anti⁃inflammatory therapeutic effects of tripterygium glycosides tablets in rheumatoid arthritis rats after long⁃term administration is related to their effects on gut microbiota.