侵染云南雾水葛的菜豆金色花叶病毒属病毒的全基因组结构特征

Molecular characterization of begomoviruses from Pouzolzia zeylanica in Yunnan, China

为明确云南雾水葛表现叶片黄化、花叶等症状的植株是否被菜豆金色黄花叶病毒属病毒侵染,本研究通过PCR扩增、克隆测序及核苷酸序列特征分析,确定样品中病原种类及其系统进化关系。结果显示,该病样中检测到雾水葛金色花叶病毒(pouzolzia golden mosaic virus, PouGMV),该分离物全基因组具有典型的菜豆金色花叶病毒属单组分病毒结构特征,与来自越南的Vietam分离物(KC857508)亲缘关系最近,相似性最高达94.6%,与PouGMV其他分离物亲缘关系相对较远,相似性在88.9%~93.2%之间。病毒alpha卫星分子检测及分析显示,该样品中检测到的alpha卫星分子,其全长核苷酸序列与云南番茄黄化曲叶alpha卫星(tomato yellow leaf curl Yunnan alphasatellite, TYLCYnA)相似性最高,达到92.3%。研究结果表明云南雾水葛中分离到PouGMV,且异源伴随TYLCYnA卫星分子。这是雾水葛金色花叶病毒伴随alpha卫星分子侵染雾水葛的首次报道。

In order to determine whether the Pouzolzia zeylanica plants with yellow and mosaic symptoms in Yunnan Province were infected by begomoviruses, PCR amplification, cloning and sequencing were conducted to determine the types of pathogens in these samples. The results showed that the pouzolzia golden mosaic virus(PouGMV) was identified from these diseased plants. The PouGMV isolate had a genome organization typical of monopartite begomoviruses and was most closely related to the PouGMV(KC857508) identified from Vietnam(with the highest sequence identity of 94.6%), and relatively distant from other PouGMV isolates(with the highest sequence identity of 88.9%-93.2%). Alphasatellite detection showed that the alphasatellite molecule was also identified from these samples, and its full-length nucleotide sequences were most similar to tomato yellow leaf curl Yunnan alphasatellite(TYLCYnA), with the highest sequence identity of 92.3%. The results of our study showed that PouGMV was isolated from Pouzolzia zeylanica plants, and heterologously associated TYLCYnA satellite molecules. This is the first report of PouGMV associated alphasatellite infecting Pouzolzia zeylanica plants.