解淀粉芽胞杆菌ZF57微粉剂的研制及对黄瓜棒孢叶斑病的防治效果

Preparation of Bacillus amyloliquefaciens ZF57 micropowder and its control effect on cucumber coryneform leaf spot

采用单因素试验和响应面分析等方法优化解淀粉芽胞杆菌ZF57的发酵工艺。得到解淀粉芽胞杆菌ZF57发酵的最优条件为山梨醇10 g·L^(-1)、棉籽饼粉17 g·L^(-1)、NaH_(2)PO_(4)0.5 g·L^(-1)、Na;HPO;0.4 g·L^(-1),初始pH 7.0、装液量80 mL/250mL三角瓶、接种量2.1%、培养温度30℃、转速180 r·min^(-1)、发酵时间32 h,此时含菌量达到3.8×10^(8)CFU·mL^(-1),较优化前提高了80%。明确了解淀粉芽胞杆菌微粉剂的最佳配方为解淀粉芽胞杆菌母粉50%,白炭黑10%,分散剂木质素磺酸钠1%,表面活性剂十二烷基硫酸钠4%,保护剂羧甲基纤维素钠1%,硅藻土补足至100%。该制剂含菌量为3.26×10^(9)CFU·mL^(-1),平均粒径8.31μm,分散指数98.16%,浮游性指数85.37,含水率1.42%,坡度角68°,各项检测结果均符合标准。解淀粉芽胞杆菌ZF57微粉剂对黄瓜棒孢叶斑病的田间防治效果高达97.12%。

Single factor test and response surface analysis were used to optimize the fermentation process of Bacillus amyloliquefaciens ZF57. Results showed that the optimal medium and culture condition for strain ZF57 were as follows: sorbitol 10 g·L^(-1), cottonseed cake powder 17 g·L^(-1), NaH_(2)PO_(4)0.5 g·L^(-1)、Na;HPO;0.4 g·L^(-1), pH 7.0, 80 mL medium in 250 mL flask, inoculum concentration 2.1%, 30 ℃, 180 r·min^(-1), 32 h. Under the optimized condition, the yield of strain ZF57 reached to 3.8 × 10^(8) CFU·mL^(-1), increased by 80% in comparison with the initial fermentation process. Screened a nutritional auxiliary and the optimum formulation of Bacillus amyloliquefaciens ZF57 micropowder. 50% of satrin ZF57 mother powder as active ingredient, 10% silica as second carrier, 1% sodium ligninsulfonate as dispersant, 4% sodium dodecyl sulfate as surfactant, 1% carboxymethylcellulose as protector, diatomite makes up to 100%. The B. amyloliquefaciens ZF57 micropowder has a particle size of 8.31 μm and a dispersion index of 98.16%. The planktonic index is 85.37, the water content is 1.42%, The slope angle is 68°. These results were in line with formulation standard. And the control effect of Bacillus amyloliquefaciens ZF57 powder on cucumber coryneform leaf spot was as high as 97.12%.