草莓根腐病的Dactylonectria病原菌鉴定

Identification of pathogen causing strawberry Dactylonectria root rot

0引言草莓(Fragaria×ananassa Duch.)为蔷薇科草莓属多年生草本植物[1],是北京市特色优势果品之一,为北京农民增收,区县经济发展,发挥了重要作用。引起草莓根腐病的病原菌种类众多,是较难防治的草莓病害。据报道,引起草莓根腐病常见的病原菌有镰刀菌属(Fusarium)、刺盘孢属(Colletotrichum)、疫霉属(Phytophthora)和丝核菌属(Rhizoctonia)[1]。

From 2018 to 2019, we carried out the occurrence and pathogen identification of strawberry root rot in Beijing. Samples were collected for tissue isolation and purification, and three single-spore strains(JZB3310013, JZB3310014 and JZB3310015) were obtained. According to the morphological characteristics, three strains were primarily identified as Dactylonectria sp.. The phylogenetic tree showed that JZB3310013 and JZB3310014 were clustered with Dactylonectria alcacerensis(CBS 129087), and JZB3310015 was clustered with D. torresensis(CBS 129086). Based on both morphological characters described and phylogenetic analysis, JZB3310013 and JZB3310014 were identified as D. alcacerensis, and JZB3310015 were identified as D. torresensis. The assay of Koch’s postulates confirmed that both D. alcacerensis and D. torresensis were the pathogen of this disease. To our knowledge, this is the first report of D. alcacerensis causing root rot on strawberry worldwide, and D. torresensis causing root rot on strawberry in China.