LRP8在结直肠癌中的表达及临床意义

The expression and clinical significance of LRP8 in colorectal cancer

目的探讨低密度脂蛋白受体相关蛋白8(LRP8)在结直肠癌组织中的表达情况及其与临床病理特征之间的关系,为结直肠癌患者的诊治及预后评估提供理论依据。方法收集湖南省人民医院2020年1月1日至2020年9月1日住院的结直肠癌患者手术切除标本(术前未行放化疗及免疫治疗等辅助治疗措施)和相应的癌旁正常组织45例,采用免疫组化检测LRP8在结直肠癌及癌旁正常组织中的表达,并通过单因素logistic回归分析、Spearman相关性分析明确LRP8与结直肠癌患者临床特征的关系。结果免疫组化结果显示45例结直肠癌组织中LRP8蛋白高表达37例(82.22%),低表达8例(17.78%);45例癌旁正常组织中LRP8蛋白高表达17例(37.78%),低表达28例(62.22%);LRP8蛋白在结直肠癌组织和其癌旁正常组织中的表达差异具有统计学意义(P<0.05)。χ^(2)检验发现LRP8蛋白的表达与结直肠癌的分期、神经侵犯、血管侵犯、癌胚抗原(CEA)、低密度脂蛋白有关(均P<0.05);与性别、年龄、肿瘤分化程度、Ki67、糖类抗原199(CA199)、淋巴结转移、甘油三酯、总胆固醇、高密度脂蛋白无关(均P>0.05);经logistic回归分析发现,TNM分期、血管侵犯、神经侵犯及血清CEA、低密度脂蛋白水平均为结直肠癌患者LRP8高表达的影响因素(均P<0.05)。ApoB与肿瘤分期(r_(s)=-0.382)、淋巴结转移(r_(s)=-0.316)、血管侵犯(r_(s)=-0.311)存在弱的负相关(均P<0.05)。结论LRP8蛋白在结直肠癌组织中表达增加,其表达增加与结直肠癌的分期、神经血管侵犯、低密度脂蛋白水平存在相关性;LRP8高表达的患者,其肿瘤分期更差、更易并发神经及血管侵犯。

Objective To investigate the expression of low density lipoprotein receptor associated protein 8(LRP8)in colorectal cancer and its correlation with clinicopathological features.Methods The surgical specimens of colorectal cancer patients hospitalized in Hunan Provincial People's Hospital from January 1,2020 to September 1,2020(without adjuvant treatment measures such as radiotherapy,chemotherapy and immunotherapy)and 45 corresponding adjacent normal tissues were collected.Immunohistochemistry was used to detect the expression of LRP8 in colorectal cancer and paracancer normal tissues.The relationship between LRP8 and clinical characteristics of colorectal cancer patients was determined by univariate logistic regression analysis and Spearman correlation analysis.Results Immunohistochemical results showed that LRP8 protein was highly expressed in 37 cases(82.22%)and low expressed in 8 cases(17.78%)of 45 colorectal cancer tissues.LRP8 protein was highly expressed in 17 cases(37.78%)and low expressed in 28 cases(62.22%).The expression of LRP8 protein in colorectal cancer tissues and adjacent normal tissues was significantly different(P<0.05).Chi-square test showed that LRP8 protein expression was correlated with colorectal cancer stage,perineural invasion,vascular invasion,carcinoembryonic antigen(CEA)and low density lipoprotein(LDL)(all P<0.05),but not correlated with gender,age,tumor differentiation,Ki67,CA199,lymph node metastasis,triglyceride,total cholesterol and high density lipoprotein(all P>0.05).Logistic regression analysis showed that tumor node metastasis(TNM)stage,vascular invasion,nerve invasion,serum CEA and LDL levels were the influencing factors of LRP8 overexpression in patients with colorectal cancer(all P<0.05).There was a weak negative correlation between ApoB and tumor stage(r_(s)=-0.382),lymph node metastasis(r_(s)=-0.316)and vascular invasion(r_(s)=-0.311)(all P<0.05).Conclusions Our results indicate that there is a higher expression of LRP8 in colorectal cancer tissues.The expression of LRP8 correlates with the staging of the colorectal cancer,neurovascular invasion,CEA,and low density lipoprotein.Patients with high expression of LRP8 have worse tumor stage and are more likely to have concurrent neurologic and vascular invasion.