氧化石墨烯对体外培养人支气管上皮细胞周期和凋亡的影响

Effects of Graphene Oxide on Cell Cycle and Apoptosis of Human Bron-chial Epithelial Cells in Vitro

随着氧化石墨烯(graphene oxide,GO)在生物电子学、生物传感器和生物医学方面的广泛应用,其对人体及其他生物体的安全构成潜在威胁,引发的健康风险正受到广泛关注。本研究使用流式细胞术,免疫印迹技术检测了GO纳米片对人支气管上皮细胞BEAS-2B胞内活性氧(ROS)、细胞周期、细胞凋亡的影响。结果显示GO暴露剂量>50 mg·L^(-1)时能够引起胞内ROS增多,当暴露剂量达到100 mg·L^(-1)能够引起DNA分子损伤,导致明显的细胞周期异常,并激活了Caspase-3介导的凋亡信号通路,诱发细胞凋亡。本研究从分子生物学水平上揭示了GO对BEAS-2B细胞的损伤机制,为其诱发的呼吸系统疾病的治疗提供了有价值的理论依据。

With the wide application of graphene oxide(GO)in bioelectronics,biosensors and biomedicine,the health risks of GO on human body and other organisms are attracting extensive attention.In this study,cell cycle,apoptosis and intracellular reactive oxygen species(ROS)production in GO nano-sheets treated human bronchial epithelial cells(BEAS-2B cells)were detected by flow cytometry and Western blot.The results showed that GO could increase intracellular ROS production at dose>50 mg·L^(-1),lead to significant cell cycle arrest,and activate Caspase-3 signaling pathway to trigger cell apoptosis at dose of 100 mg·L^(-1).This study revealed the molecular mechanisms of GO-induced oxidative damage in BEAS-2B cells,and provided valuable theoretical basis for the treatment of respiratory diseases induced by GO.