2019-2020年重庆市大型养猪场伪狂犬病毒野生毒株感染的流行病学调查

Epidemiological investigation of pseudorabies virus wild virus infection in some large pig farms in Chongqing from 2019 to 2020

目的调查2019-2020年重庆市部分地区大型养猪场猪伪狂犬病病毒(PRV)野生毒株感染流行情况,为防控措施的确定提供参考。方法2019-2020年在重庆市32家大型养猪场采集3640份猪血清,采用酶联免疫吸附试验(ELISA)检测PRV-gE和PRV-gB,分析PRV野生毒株感染情况。结果32家大型养猪场共采集猪血清3640份,ELISA检测PRV-gE抗体阳性率为37.40%(1362/3640),PRV-gB抗体阳性率为93.20%(848/3640)。公猪和母猪、哺乳/保育仔猪、育肥猪及后备猪PRV-gE抗体阳性率分别为35.80%、44.20%、33.50%、29.20%、48.90%和32.70%,不同公猪和母猪、哺乳和保育仔猪、育肥猪及后备猪之间PRV-gE抗体阳性率比较,差异具有统计学意义(P<0.05)。各地区的养猪场PRV感染率不同,忠县PRV感染率最高,为52.20%,与其他县区感染率相比差异有统计学意义(P<0.05)。第四季度猪PRV-gE抗体阳性率为43.00%,与其它季度相比差异具有统计学意义(P<0.05)。结论重庆地区部分规模化养猪场猪PRV野生毒株感染率高,相关部门应采取针对性措施加强PRV野生毒株感染的控制与净化。

Objective Large pig farms in some areas of Chongqing from 2019 to 2020 were selected as the research object to investigate the infection of porcine pseudorabies virus(PRV)wild virus to determine relevant virus prevention and control measures in combination with the research results.Methods 3640 sera collected from 32 large pig farms in Chongqing were investigated by enzyme-linked immunosorbent assay(ELISA).The results were statistically analyzed.Results 3640 pig sera were collected from 32 large pig farms.The positive rate of PRV Ge antibody by ELISA was 37.40%(1362/3640),and the positive rate of PRV GB antibody was 93.20%(848/3640).The positive rates of PRV Ge antibody in boars and sows,lactating/nursing piglets,fattening pigs and reserve pigs were 35.80%,44.20%,33.50%,29.20%,48.90%and 32.70%respectively.There was significant difference in the positive rates of PRV Ge antibody between boars and sows,lactating and nursing piglets,fattening pigs and reserve pigs(P<0.05).The positive rate of serum PRV Ge antibody of pigs in Zhongxian County and Wuxi County was more than 50.00%,which was significantly different from that of pigs in farms in Yunyang County,Fengjie County,Chengkou County,Fengdu County,Youyang Tujia and Miao Autonomous County and Wushan County(all P<0.05).The positive rate of serum PRV GB antibody of pigs in farms in Youyang Tujia and Miao Autonomous County was lower,which was statistically significant compared with that in farms in other areas(P<0.05)The positive rate of PRV Ge antibody in pig serum in the fourth quarter of each year was 43.00%,which was statistically significant compared with other quarters(P<0.05);There was no significant difference in the positive rate of PRV GB antibody between the four quarters(P>0.05).Conclusion In Chongqing,some large-scale pig farms face tremendous pressure of pseudorabies virus infection,and relevant departments need to strengthen control and purification.