食源性沙门氏菌血清分型及分子分型分析

Serotyping and molecular typing analysis of foodborne Salmonella

目的:对贵阳市2015年至2020年33株食源性沙门氏菌进行血清分型和PFGE分子分型,评价不同血清型沙门菌的亲缘性,为贵阳市沙门菌感染的防控提供参考数据。方法:对目标沙门氏菌进行血清凝集确定其血清型,并使用脉冲场凝胶电泳(Pulsed field gel electrophoresis,PFGE)进行分子分型。结果:血清分型共分出5种血清型,其中优势血清型为鼠伤寒沙门(46%)与德尔卑沙门(21%)。分子分型33株菌株中4株分型失败,剩余29株共获得24种带型,相似性达100%的有4组(有一组为不同年份肠炎沙门),鼠伤寒优势带型为P15(25%),德尔卑沙门氏菌优势带型为P20(33.3%)。结论:本实验沙门菌PFGE分型总体呈多态性,不同年份肠炎沙门分型一致提示存在污染源清除不彻底问题。

Objective:To conduct serological and PFGE molecular typing of 33 foodborne salmonella strains in Guiyang from 2015 to 2020,and to evaluate the affinity of different serotypes of Salmonella,so as to provide reference data for the prevention and control of Salmonella infection in Guiyang.Methods:Serotypes of salmonella were determined by serum agglutination,and molecular typing was performed by pulsed field gel electrophoresis(PFGE).Results:Five serotypes were identified,among which the dominant serotypes were Salmonella typhimurium(46%)and Delpersamen(21%).Among the 33 strains,4 strains failed in molecular typing,and 24 bands were obtained from the remaining 29 strains.Four groups with 100%similarity(one group was Salmonella enteritis in different years),the dominant band type of typhus was P15(25%),and the dominant band type of Salmonella delpy was P20(33.3%).Conclusion:The PFGE typing of Salmonella in this study was generally polymorphic,and the consistent typing of salmonella enteritis in different years suggested incomplete removal of pollution sources.