改良型平板流动小室的研制及其在病理血管条件下血小板黏附功能检测中的应用

Development of Improved Parallel Plate Flow Chamber and Its Application in the Measurement of Platelet Adhesion Under Pathological Vascular Conditions

目的研制一种合适的体外模型,用以观察病理血管条件(包括狭窄、血流紊乱、静脉瓣膜受损等)下血小板在血管壁的黏附功能。方法本研究设计了一种改良的平板流动小室装置,以不同剪切力、不同血管管腔直径对生理及病理状态下血小板的黏附进行了观测,同时对流经小室但未黏附的血小板进行聚集功能检测。结果带有荧光标记血小板的外周血以正常静脉流速流过胶原包被的流动小室,约5~8 min可观察到血小板呈片状黏附于管壁,最大血小板覆盖面积约60%;使用针对血小板黏附功能的抑制剂SZ-2能显著抑制血小板的黏附。同样的血小板在局部狭窄管腔和存在血管分支的管腔中黏附面积增大;流经局部狭窄管腔的血小板聚集率明显高于流经正常管腔的血小板。结论改良型平板流动小室能较好地模拟生理和血管病理条件下的血流状态,对于检测血小板功能、监测抗血栓药物疗效有较高的应用价值。

Objective Develop a suitable in vitro model to observe the adhesion of platelets on the vessel wall under pathological vascular conditions(including stenosis,blood flow disturbance,venous valve damage,etc.).Methods In this study,an improved parallel flow chamber device was designed to detect the adhesion of platelets under physiological and pathological conditions with different shear rates and different vessel diameters.At the same time,the aggregation of platelets that flow through the chamber but not adhered was measured.Results Peripheral blood with fluorescently labeled platelets flows through the collagen-coated flow chamber at a normal flow rate.Platelets adhered to the tube wall can be detected in about 5~8 minutes.The maximum platelet coverage area is about 60%;the inhibitor SZ-2 can significantly inhibit platelet adhesion.The same platelet adhesion area increases in the local stenosis lumen and the lumen with blood vessel branches;the platelet aggregation rate flowing through the local stenosis lumen is significantly higher than that of the platelets flowing through the normal lumen.Conclusion The improved parallel flow chamber can simulate the blood flow state under physiological and vascular pathological conditions,and has application value for researching platelet function and monitoring the efficacy of antithrombotic reagents.