二噁英暴露对儿童早期类固醇激素的影响

Effects of dioxin exposure on steroid hormones in early childhood

为了阐明在儿童重要发育阶段二噁英对其内分泌的干扰.本研究于2010年和2011年,招募二噁英暴露地区37对母婴和非暴露地区44对母婴,运用气相色谱-质谱法测定母乳中二噁英含量,运用液相色谱-串联质谱法测定儿童唾液类固醇激素含量.结果显示,暴露地区母乳中二噁英含量(Mean=11.0 pg·lipid^(-1))明显高于非暴露地区(Mean=3.4 pg·lipid^(-1)).暴露地区男童1岁时唾液中脱氢表雄酮(DHEA)含量与二噁英同系物中2,3,7,8-TeCDD(β=0.4235;P=0.0395)、1,2,3,7,8-PeCDD(β=0.4742;P=0.0190)、1,2,3,4,7,8-HxCDD(β=0.4949;P=0.0140)和1,2,3,6,7,8-HxCDD(β=0.4745;P=0.0327)以及TEQ PCDD(β=0.6566;P=0.0005)和TEQ PCDD/DF(β=0.6137;P=0.0014)呈正相关(P<0.05),5岁时与二噁英同系物1,2,3,7,8-PeCDD(β=-0.5925;P=0.0057)、1,2,3,6,7,8-HxCDD(β=-0.6350;P=0.0026)和1,2,3,7,8,9-HxCDD(β=-0.5623;P=0.0108)呈负相关(P<0.05),而女童1岁时与二噁英呈负相关(P<0.05),但5岁时无相关性(P>0.05).综上所述,围产期二噁英暴露对儿童早期DHEA含量影响具有性别差异.

The present study was to follow up and more clearly elucidate this endocrine disruption by dioxin during the important developmental stages of children.In 2010 and 2011,37 mother–infant pairs in the exposed region and 44 pairs in the non-exposed region were enrolled in the present study.Dioxin was determined by gas chromatography-mass spectrometry.Saliva steroid hormones were determined by liquid chromatography-tandem mass spectrometry.The results showed that breast milk levels of dioxin congeners in the exposed region(Mean=11.0 pg·lipid^(-1))were significantly higher than the non-exposed region(Mean=3.4 pg·lipid^(-1)).In the exposed region,dehydroepiandrosterone(DHEA)in saliva of boys was positively correlated with 2,3,7,8-TeCDD(β=0.4235;P=0.0395),1,2,3,7,8-PeCDD(β=0.4742;P=0.0190),1,2,3,4,7,8-HxCDD(β=0.4949;P=0.0140),1,2,3,6,7,8-HxCDD(β=0.4745;P=0.0327),TEQ PCDD(β=6566;P=0.0005),and TEQ PCDD/DF(β=0.6137;P=0.0014)at the age of 1(P<0.05)and negatively correlated with 1,2,3,7,8-Pe CDD(β=-0.5925;P=0.0057),1,2,3,6,7,8-Hx CDD(β=-0.6350;P=0.0026),and 1,2,3,7,8,9-Hx CDD(β=-0.5623;P=0.0108)at the age of 5(P<0.05).An increase in maternal dioxins related to increased DHEA levels in male salivary samples.However,an increase dioxin related to decreased DHEA levels in female salivary samples at the age of 1(P<0.05),but not at the age of 5(P>0.05).Our founding suggested that the effect of perinatal dioxin exposure on children’s early DHEA content has gender differences.