稀有鮈鲫体内多种神经化学物质的同时测定

Simultaneous determination of multiple neurochemicals in Chinese rare minnow

本文建立了一种可以同时检测稀有鮈鲫体内24种神经化学物质的分析方法.该方法采用QuEChERS技术对样品进行提取和净化,待测物采用超高效液相色谱-串联质谱法(UPLC-MS/MS)分析,利用同位素内标法进行定量.该方法仪器检测限(IDL)范围为0.01—0.06 ng·mL^(-1),方法定量限(MQL)范围为0.28—1.97 ng·g^(-1).在两种浓度添加水平下,基质效应处于60.6%—129.0%之间,相对回收率处于66.8%—116.7%之间,仪器日内偏差≤8.3%,仪器日间偏差≤10.9%,方法相对标准偏差≤10.8%.结果表明,该方法准确可靠,满足鱼体中神经化学物质定量分析要求.

An analytical method was developed to simultaneously detect 24 neurochemicals in Chinese rare minnow(Gobiocypris rarus).In the method,QuEChERS is employed to extract and purify the sample.The neurochemicals are then analyzed and quantified by ultra-high performance liquid chromatography–tandem mass spectrometry(UPLC-MS/MS)based on isotope internal standard method.The instrument detection limits(IDL)range from 0.01 to 0.06 ng·mL^(-1),and the method quantification limits(MQL)are in the range of 0.28 to 1.97 ng·g^(-1).The matrix effects are between 60.6%and 129.0%at the two spiking levels.The relative recoveries are in the range of66.8%to 116.7%.The instrument intraday deviation are less than or equal to 8.3%,the instrument daily deviation are less than or equal to 10.9%,the relative standard deviations of method are less than or equal to 10.8%.The results show that the method is accurate and reliable,and meets the requirement of neurochemicals detection in fish.