摘要
目的:探究急性胰腺炎(AP)患者外周血长链非编码RNA H19(lncRNA H19)表达与辅助性T细胞17(Th17)/调节性T细胞(Treg)平衡的关系。方法:以2019年9月—2021年9月收治的91例AP患者为研究对象,根据患者病情程度分为轻度急性胰腺炎(MAP)34例,中度急性胰腺炎(MSAP)31例,重度急性胰腺炎(SAP)26例;另选取75例健康体检者为对照组。各组外周血lncRNA H19水平检测采用实时荧光定量PCR(qRT-PCR)法;Th17、Treg细胞水平采用流式细胞术检测;外周血IL-17、IL-6、IL-10、转化生长因子-β(TGF-β)水平利用酶联免疫吸附法检测。Pearson相关性分析外周血lncRNA H19与Th17/Treg及其相关细胞因子的关系。结果:各组之间检测降钙素原、C反应蛋白、总胆固醇、三酰甘油、高密度脂蛋白、低密度脂蛋白水平、急性生理学和慢性健康状况评分比较均差异有统计学意义(P>0.05)。与对照组相比,AP组患者外周血lncRNA H19、Th17细胞比例、Th17/Treg比值、IL-17、IL-6水平均明显升高,且随疾病进展而显著上升,而Treg细胞比例、IL-10、TGF-β水平明显下降,且随疾病加重而显著下降(P<0.05)。Pearson相关性分析结果显示,AP组患者外周血lncRNA H19水平与Th17、Th17/Treg、IL-17、IL-6呈显著正相关(P<0.05),与Treg、IL-10、TGF-β呈显著负相关(P<0.05)。结论:AP患者外周血lncRNA H19表达水平及Th17/Treg比值均增加,其机制可能是lncRNA H19通过影响Th17/Treg平衡而参与AP的发生、发展。
Objective:To explore the relationship between the expression of long non-coding RNA H19(lncRNA H19)in peripheral blood of patients with acute pancreatitis(AP)and the balance of helper T cell 17(Th17)/regulatory T cell(Treg).Methods:Ninety-one patients with AP who accepted by our hospital from September 2019 to September 2021 were gathered as the research objects.According to the severity of the patients,they were separated into 34 cases of mild acute pancreatitis(MAP),31 cases of moderate acute pancreatitis(MSAP)and 26 cases of severe acute pancreatitis(SAP),and 75 healthy subjects were gathered as control group.The level of lncRNA H19 in peripheral blood was measured by real-time quantitative PCR(qRT-PCR)method;the levels of Th17 and Treg cells in each group were measured by flow cytometry;the levels of interleukin-17(IL-17),interleukin-6(IL-6),interleukin-10(IL-10)and transforming growth factor-β(TGF-β)in peripheral blood were measured by enzyme-linked immunosorbent assay(ELISA).The relationship between peripheral blood lncRNA H19 and Th17/Treg and its related cytokines was analyzed by Pearson correlation analysis.Results:There were significant differences in PCT,CRP,TC,TG,HDL-C,LDL-C,and APACHEⅡamong patients in each group(P>0.05).Peripheral blood lncRNA H19,Th17 cell ratio,Th17/Treg ratio,IL-17,IL-6 levels in AP patients increased greatly than control group,and increased greatly with disease progression,while Treg cell ratio,IL-10,TGF-βlevels decreased greatly,and decreased greatly with the aggravation of the disease(P<0.05).The results of Pearson correlation analysis showed that the level of lncRNA H19 in peripheral blood of AP patients was greatly directly correlated with Th17,Th17/Treg,IL-17,and IL-6(P<0.05),and was greatly inversely correlated with Treg,IL-10,and TGF-β(P<0.05).Conclusion:The expression level of lncRNA H19 and the ratio of Th17/Treg in peripheral blood of AP patients are increased,and the mechanism may be that lncRNA H19 participates in the occurrence and development of AP by affecting the Th17/Treg balance.
作者
徐晨
何精选
石珍
徐鹏
XU Chen;HE Jingxuan;SHI Zhen;XU Peng(Department of Emergency,General Hospital of the Yangtze River Shipping,Wuhan,430019,China)
出处
《临床急诊杂志》
CAS
2022年第5期316-320,326,共6页
Journal of Clinical Emergency