黄花菜抗氧化抑菌活性的筛选以及化学成分分析

Screening of antioxidant and antibacterial activities and analysis of chemical constituents of Hemerocallis citrina Baroni

试验采用2,2-联苯基-1-苦基肼基法(DPPH)、铁离子还原法(FRAP)评价黄花菜根和花不同萃取部位的抗氧化活性,采用微量稀释法评价黄花菜根和花不同萃取部位的抑菌活性,通过超高效液相色谱串联四极杆飞行时间质谱(UPLC-Q-TOF-MS)分析具有显著活性的萃取部位的化学成分。结果显示:FRAP结果显示根乙酸乙酯萃取部位(6.88 mmol/g)总抗氧化能力强于对照组(6.59 mmol/g),其余萃取部位均未表现明显的总抗氧化能力。DPPH结果显示,在样品溶液浓度为0.4~1.0 g/L时,黄花菜的根和花乙酸乙酯萃取部位的DPPH自由基的清除能力明显强于没食子酸丙酯(PG),与维生素C (VC)无差异,清除率均大于90.8%。经测定,根乙酸乙酯、正丁醇萃取部位和花二氯甲烷、正丁醇萃取部位基本无明显的抑菌效果,其余部位均表现一定的抑菌效果,但最小杀菌浓度(MBC)值均≥10 g/L。研究表明,具有活性的部位经UPLC-Q-TOF-MS检测,所得MS以及MS/MS数据结合质谱裂解规律分析,初步鉴定出39个化学成分,主要为酚酸类、黄酮类及蒽醌类化合物。

In the study, DPPH method and FRAP method were used to evaluate the antioxidant activities of different extraction parts of the roots and flowers of Hemerocallis citrina Baroni. Microdilution method recommended by CLSI was used to evaluate the antibacterial activity of different extracts the roots and flowers of Hemerocallis citrina Baroni, and UPLC-Q-TOF-MS was used to analyze the chemical components of the extracts with significant activity. The results showed that the total antioxidant capacity of ethyl acetate extract(6.88 mmol/g) was stronger than that of control group(6.59 mmol/g), and no obvious FRAP total antioxidant capacity was observed in other extracts. The DPPH results showed that when the concentration of sample solution was 0.4~1.0 g/L, the DPPH free radical scavenging capacity of the root and flower ethyl acetate extracts was significantly stronger than that of PG, which was not different from that of VC, and the scavenging rates were all greater than 90.8%. The results showed that the ethyl acetate and n-butanol extract of root and dichloromethane and n-butanol extract of flower had no obvious antibacterial effect. Others showed certain antibacterial effect, but the minimum bactericidal concentration(MBC) values were greater than 10 g/L. The experiment indicates that the samples with obvious activity were detected by UPLC-Q-TOF-MS and a total of 39 chemical components mainly including phenolic acids, flavonoids and anthraquinones were preliminarily identified by MS and MS/MS data combined with fragmentation pathways.