常压室温等离子体(ARTP)诱变介导的赤藓糖醇生产菌的筛选与优化

Screening and optimization of erythritol-producing bacteria mediated by atmospheric and room temperature plasma (ARTP) mutagenesis

赤藓糖醇是一种天然多元醇类甜味剂,具有热量较低、无致龋齿性、口感佳、非吸湿性以及对糖尿病患者安全等优点,已成为大多数保健食品成分的优先选择。与化学合成方法相比,赤藓糖醇的微生物发酵生产过程温和,易于控制,具有更多的生产优势。在本研究中,笔者从蜂巢、土壤和花粉等物质中筛选出了一株赤藓糖醇高产菌株,鉴定其为Moniliella sp.MU1。用常压室温等离子体(ARTP)生物育种系统对该菌株进行突变并从中挑选出4种菌株进行发酵并优化发酵条件,结果发现:不同种类的碳源、葡萄糖浓度和金属离子条件都会影响赤藓糖醇的产量,高浓度的葡萄糖有利于赤藓糖醇的生产;同时,当提供300 g/L葡萄糖和25 mmol/L Ca^(2+)时,菌株YE-A27的赤藓糖醇产量达到97.6 g/L。

Erythritol is a natural polyol sweetener.Because of its low-calorie value,non-cariogenic properties,good taste,non-hygroscopicity and safety for diabetics,it has become the new favorite of health food ingredients.Compared with chemical synthesis methods,the microbial fermentation production process of erythritol is mild,easy to control,and has more production advantages.In this study,we screened a high-yield erythritol strain from honeycomb,soil,pollen and other materials,and identified as Moniliella sp.MU1.The strain was mutated using the atmospheric and room temperature plasma(ARTP) biological breeding system.Four strains were selected for fermentation and fermentation conditions were optimized.Different carbon sources,glucose concentrations and metal ion conditions affected the erythritol production,and high-concentration of glucose was conducive to the production of erythritol.At the same time,when 300 g/L glucose and 25 mmol/L Ca^(2+) were provided,the erythritol output of strain YE-A27 was the highest,reaching 97.6 g/L.