基于HPLC多成分定量结合外观性状的金银花质量评价与等级标准的制定

Quality Evaluation and Grading Standard of Lonicera Japonica Flos Based on HPLC Multi-Component Quantitative Analysis and Appearance Traits

目的:分析对比主产区和部分新产区金银花的差异,研究金银花药材质量的分级规律,建立广泛通用的金银花等级质量标准。方法:收集8个地区共计78批样品,测定外观性状指标,包括颜色、黄条率和枝叶率,通过高效液相色谱法测定绿原酸、异绿原酸A、异绿原酸C、总酚酸和木犀草苷含量;使用SPSS 26.0和SIMCA 14.1软件进行质量评价和等级划分。结果:8个地区金银花样品绿原酸质量分数为1.17%~3.66%,总酚酸质量分数为3.34%~6.59%,木犀草苷质量分数为0.045%~0.185%。主成分分析对比发现甘肃通渭、云南云龙地区金银花同主产区金银花存在较大差异,不同加工方式处理的金银花区分较为明显。根据综合评分结果将金银花分为4个等级。结论:部分样品不合格的主要原因为总酚酸含量不达标。主产区金银花特征相似,不同新产区的金银花质量差异较大。建立的金银花等级质量标准能够反映药材质量的差异性,为不同产地金银花药材质量评价提供参考。

Objective:To analyze and compare the differences of Lonicerae Japonicae Flos(LJF)between the major and new producing regions and explore the pattern of quality grading of LJF,thereby establishing a widely-used quality standard for LJF grading.Methods:Seventy-eight samples of LJF from 8 different regions were collected to determine the indexes of appearance traits,including color,yellow strip rate,and rate of branch and leaf.Chlorogenic acid,isochlorogenic acid A,isochlorogenic acid C,total phenolic acid content,and luteoloside content were determined by high performance liquid chromatograph(HPLC).SPSS 26.0 and SIMCA 14.1 were used to evaluate the quality and classify the grades.Results:The chlorogenic acid content,the total phenolic acid content,and the content of luteoloside of the samples in the 8 producing regions were 1.17%-3.66%,3.34%-6.59%,and 0.045%-0.185%,respectively.Principal component analysis showed that LJF in Tongwei,Gansu province and Yunlong,Yunnan province were significantly different from that in the main producing regions,and LJF treated by different processing methods varied obviously.LJF was divided into four grades based on the comprehensive scoring results.Conclusion:The main reason for the unqualified LJF samples was that the content of total phenolic acids did not meet the standard.The characteristics of LJF in the major producing regions were similar,while the quality of LJF in the new producing regions was significantly different.The established quality standards for LJF grading reflected the quality differences of LJF and provided references for the quality evaluation of LJF in different producing regions.