三明治肝细胞培养模型及其在中药肝毒性评价中的应用

Sandwich-cultured Hepatocytes and Application in Hepatotoxicity Evaluation of Chinese Medicine

随着中药的广泛使用,安全性逐渐成为影响其临床应用的关键因素之一。肝脏作为机体主要的代谢场所,是药物毒性反应主要的靶器官之一,而使用高效、简便且可靠的体外模型对中药肝毒性进行评价和预警是当前毒理学研究的热点。三明治肝细胞培养模型能表达肝细胞血窦侧与胆管侧的转运体,形成功能性胆小管网络,并具有代谢能力,是研究药物肝胆转运和胆汁淤积的重要工具。对三明治肝细胞培养模型的特点、构建、影响因素及其在中药肝毒性评价中的应用进行综述,为中药肝毒性的评价方法研究提供参考。

With the widespread application of Chinese medicine,the safety of Chinese medicine has become a key factor affecting its clinical application.As the major organ responsible for metabolism,liver is one of the main targets of drug-induced toxicity.Consequently,developing and utilizing an efficient,simple,and reliable in vitro model to screen and predict the hepatotoxicity of Chinese medicine has become a research hotspot in toxicological research.Sandwich-cultured hepatocyte(SCH)model can express drug transporters in both sinusoidal and canalicular poles to establish the bile canaliculi-like network with metabolic capability.It provides a unique tool to evaluate hepatobiliary drug transport and cholestasis.The present review was designed to summarize the features,establishment,and factors of SCH model,as well as its application in hepatotoxicity evaluation of Chinese medicine,which is expected to provide references for the research of evaluation methods of Chinese medicine hepatotoxicity.