蓝莓品种布里吉塔的组培无菌体系建立

Establishment of Sterile Tissue Culture System for Blueberry Cultivar Brigitta

【目的】探明蓝莓外植体采集时间、不同外植体、玉米素核苷(ZT)浓度对蓝莓初代培养的影响及细胞分裂素种类对组培苗继代生长的影响,为蓝莓组培无菌体系建立提供参考。【方法】以北高丛蓝莓品种布里吉塔为试验材料,依托组织培养技术建立无菌体系。【结果】蓝莓4—5月均可进行外植体采集,其中5月最佳;最佳外植体茎为中下段和茎下段;初代培养最佳培养基为1/2 MS+1/2 WPM+ZT 1.0 mg/L;与1.5 mg/L ZT相比,培养基中添加1.5 mg/L反式玉米素核苷(tZR)初代诱导新梢的继代生长更好。【结论】通过组培快繁技术,建立了蓝莓布里吉塔的高效组培无菌体系,初代培养最佳培养基为1/2 MS+1/2 WPM+ZT 1.0 mg/L,继代培养基为1/2 MS+1/2 WPM+1.5 mg/L tZR。

【Objective】The effects of the explant collection time, different explants and zeatin riboside concentration on the primary culture of blueberry and the effects of cytokinin kind on subculture of tissue culture seedlings are studied to provide a reference for the establishment of sterile tissue culture system for blueberry.【Method】Northern highbush blueberry cultivar Brigitta is used as test material and the aseptic system is established through the tissue culture technology.【Result】The explants for blueberry can be collected in April and May, and collecting in May is the best;The optimum explants include middle-lower stem and lower stem;The optimum medium for primary culture is 1/2 MS+1/2 WPM+1.0 mg/L ZT;Compared with 1.5 mg/L ZT, the subculture of new shoot inducing from primary culture grows better when adding 1.5 mg/L trans-zeatin riboside(tZR) in the medium.【Conclusion】Through tissue culture rapid propagation technology, the efficient sterile tissue culture system for Brigitta blueberry is established, and the optimum medium for primary culture is 1/2 MS+1/2 WPM+1.0 mg/L ZT, while the the optimum medium for subculture is 1/2 MS + 1/2 WPM+1.5 mg/L tZR.