大孔树脂纯化细毡毛忍冬中总黄酮工艺研究

Research on Techniques of Purifying the Total Flavonoids in Lonicera Similes Hemsl with Macroporous Resin

目的:研究大孔树脂纯化细毡毛忍冬总黄酮的工艺,为细毡毛忍冬化学成分的进一步研究奠定基础。方法:以静态吸附容量及解析率筛选树脂类型,在动态吸附条件上,对影响纯化的工艺参数条件进行筛选。结果:确定了大孔树脂纯化细毡毛忍冬提取物的工艺为:D101大孔树脂纯化细毡毛忍冬提取物,上柱药液生药浓度为0.10 g/mL,比上柱量2.74g/g(药材/干树脂)上柱吸附。结论:D101树脂综合性能较好,适用于细毡毛忍冬中总黄酮成分的分离纯化。

Objective To research on a purification process for the total flavonoids from Lonicera similes Hemsl by macmporous resin,and lay the foundation for the further research of the chemical composition of Lonicera similes Hemsl.Methods Based on the different static adsorption capacity and elution rate of total flavonoids,the technological conditions for absorption of macroporous absorptive resin were selected.Result The result indicates that the optimum purification process are as follows:D101 macroporous resin is used to purify the extract of Lonicera similes Hemsl,The sample solution concentration is 0.10 g crude drug/mL and the rate amount is 2.74 g/g crude drug dry resin.Conclusion With good comprehensive properties,D101 resin is suitable for purifying the element of total flavone from Lonicera similes Hemsl.