慢性涎腺炎不同进展阶段中S100A8/A9表达研究

Expression and Effect of S100A8 and S100A9 in Chronic Sialadenitis

目的:研究S100A8和S100A9蛋白在慢性涎腺炎组织中的表达,探讨其在慢性涎腺炎致病过程中的作用机制。方法:构建大鼠慢性下颌下腺炎模型并收集病理科人下颌下腺组织,苏木精-伊红(HE)染色观察大鼠和人慢性下颌下腺炎组织形态改变,免疫组织化学染色观察S100A8和S100A9在大鼠和人慢性下颌下腺炎组织中的表达和分布,以探究S100A8和S100A9蛋白在慢性涎腺炎进展中的作用机制。结果:随着导管结扎时间延长,大鼠下颌下腺组织中炎症细胞浸润增加,导管细胞及腺泡细胞萎缩,数量减少,并伴有胶原纤维增生;S100A8和S100A9蛋白在大鼠和人下颌下腺组织的炎症组中表达量较正常组增加。结论:S100A8和S100A9可作为慢性涎腺炎进展程度的标记蛋白,随着腺体炎症程度增加,其表达量增多。

Objective:To investigate the expression and function of S100A8 and S100A9 proteins in chronic submandibular gland sialadenitis.Methods:A rat model of chronic submandibular gland sialadenitis was constructed and human submandibular gland tissues were collected.The histomorphology of chronic sialadenitis and control submandibular glands were observed by HE staining,and the expressions of S100A8 and S100A9 in rat and human chronic submandibular gland tissue were observed by immunohistochemical staining,with aim to explore the mechanism of S100A8 and S100A9 proteins in the progression of chronic sialadenitis.Results:As the ligation time increased,the number of inflammatory cells increased in intralobular interstitium,and the ductal and acinar cells atrophied,accompanied with interstitial fibrosis hyperplasia.The expressions of S100A8 and S100A9 in sialadenitis groups of rats and human were more than those in normal groups.Conclusion:The expression of S100A8 and S100A9 increased with the development of chronic sialadenitis,which could be used as marker proteins for the progression of sialadenitis.