CysC、IL-2、C3在慢性肾小球肾病患者中的水平及相关性研究

Level and correlation of CysC,IL-2 and C3 in patients with chronic glomerular nephropathy

目的 本研究分析胱抑素C(cystatin C,CysC)、白细胞介素2(interleukin-2,IL-2)、C3在慢性肾小球肾病患者中的水平及相关性,旨在为临床上此病的诊断提供参考。方法 选取慢性肾小球肾病患者138例作为研究组,另外选取于行健康体检的健康志愿者80例作为对照组。检测患者血清CysC、IL-2、C3水平,分析其在2组中的表达差异及在患者疾病不同严重程度中的表达差异,并分析三者之间相关性。结果 与对照组相比,研究组CysC水平升高,IL-2、C3水平降低(P<0.05)。ROC曲线显示,与CysC、IL-2、C3单项诊断相比,三项联合对慢性肾小球肾病的诊断价值较高(P<0.05)。单因素分析显示,慢性肾小球肾病的发生与患者性别、年龄、血尿酸、合并症无关,差异无统计学意义(P>0.05);慢性肾小球肾病的发生与患者吸烟史、饮酒史、是否肥胖、CysC、IL-2、C3水平相关(P<0.05)。相关性分析显示,CysC与肾功能损害(renal function impairment,eGFR)正相关(r=0.404,P<0.001),IL-2与eGFR负相关(r=-0.375,P<0.001),C3与eGFR负相关(r=-0.504,P<0.001)。将慢性肾小球肾病发生作为因变量,将单因素分析的有意义变量作为自变量,纳入二分类多因素Logistic回归模型分析,结果显示,CysC、IL-2、C3为影响慢性肾小球肾病发生的因素(P<0.05)。CysC与IL-2负相关(r=-0.250,P=0.003);CysC与C3负相关(r=-0.373,P=0.001);IL-2与C3正相关(r=0.232,P=0.006)。结论 慢性肾小球肾病患者血清中CysC水平升高,IL-2、C3水平降低,且与患者肾功能损害相关,临床可根据其水平早期诊断评估此病。

Objective To analyze the level and correlation of Cystatin C(CysC),interleukin-2(IL-2)and C3 in patients with chronic glomerular nephropathy and to provide reference for the clinical diagnosis of this disease.Methods A total of 138 patients with chronic glomerular nephropathy were selected as the research group,and 80 healthy physical examinees in our hospital as the control group.Serum CysC,IL-2 and C3 levels were detected,and their expression differences in the two groups and in patients with different severities of disease were analyzed.The correlation among the three was analyzed.Results Compared with the control group,CysC levels were higher,while IL-2 and C3 levels were lower in the research group(P<0.05).The ROC curve showed that combined detection of CysC,IL-2 and C3 had a higher diagnostic value for chronic glomerular nephropathy than CysC,IL-2 and C3 alone(P<0.05).Univariate analysis showed that the incidence of chronic glomerular nephropathy was not related to the patient's gender,age,serum uric acid,and comorbidities,with no significant difference(P>0.05),but was related to smoking history,drinking history,obesity,CysC,IL-2 and C3 levels(P<0.05).Correlation analysis showed that CysC was positively correlated with renal function damage(eGFR)(r=0.404,P<0.001);IL-2 was negatively correlated with eGFR(r=-0.375,P<0.001);C3 was negatively correlated with eGFR(r=-0.504,P<0.001).Chronic glomerular nephropathy served as dependent variables,and significant variables of univariate analysis as independent variables were incorporated into the biclassification multi factor Logistic regression model,which showed that CysC,IL-2,and C3 were influencing factors of the occurrence of chronic glomerular nephropathy(P<0.05).CysC was negatively correlated with IL-2(r=-0.250,P=0.003);CysC was negatively correlated with C3(r=-0.373,P=0.001);IL-2 was positively correlated with C3(r=0.232,P=0.006).Conclusion Serum CysC levels are increased,while IL-2 and C3 levels are decreased in patients with chronic glomerular nephropathy,which is related to eGFR of the patients.The disease can be diagnosed and evaluated early based on their level.