盐与干旱胁迫对10份红豆草种质萌发影响的研究

Effects of Salt Stress and Drought Stress on Seed Germination of 10 Onobrychis viciaefolia Germplasms

为筛选耐盐性和抗旱性较优的红豆草种质资源,以10份来源不同的红豆草种质为材料,采用不同浓度的NaCl(0、50、100、200、300、400、500 mmol/L)以及不同浓度的PEG-6000(0、5%、15%、25%、35%、45%)模拟胁迫条件进行种子萌发试验,测定发芽率、发芽势、发芽指数和活力指数的相对指标。通过模糊数学隶属函数法对红豆草种质在种子萌发期的耐盐性和抗旱性进行综合评价,其耐盐性强弱依次为H 06、H 02、H 05、H 07、H 09、H 08、H 10、H 01、H 04、H 03,抗旱性强弱依次为H 06、H 05、H 01、H 02、H 10、H 07、H 08、H 09、H 04、H 03,其中H 06的耐盐性和抗旱性均优于其他红豆草种质。研究结果可为今后杂交创制新种质提供遗传材料。

10 Onobrychis viciaefolia germplasms of different sources were selected as experimental materials for screening the better Onobrychis viciaefolia materials with both better salt tolerance and drought resistance.The different concentrations of NaCl with 0,50,100,200,300,400 and 500 mmol/L and PEG-6000 with 0%,5%,15%,25%,35%and 45%were used to simulate the stress conditions for seed germination experiment.Relative germination rate,relative germination potential,relative germination index and relative germination vigor index were measured.The method of fuzzy mathematical affiliation function was used for comprehensive evaluation of the salt tolerance and drought resistance of Onobrychis viciaefolia germplasms in the seed germination stage.The 10 germplasms were sorted according to their salt tolerance and drought resistance from strong to weak were H 06,H 02,H 05,H 07,H 09,H 08,H 10,H 01,H 04,H 03 and H 06,H 05,H 01,H 02,H 10,H 07,H 08,H 09,H 04,H 03,of which the salt tolerance and drought resistance of H 06 were better than other Onobrychis viciaefolia germplasms.The study results could provide inheritance materials for creating new hybrid germplasm in the future.