三种芽孢杆菌菌剂对烤烟育苗效果的影响

Effects of Three Bacillus Agents on Seedling Growth of Flue-cured Tobacco

为深入了解微生物菌剂在烟草育苗期的作用,探究其对烟草幼苗根系生长发育和烤后烟叶品质的影响,在烟草育苗时期,向漂浮育苗营养液中分别添加3种微生物菌剂:侧孢芽孢杆菌、枯草芽孢杆菌和地衣芽孢杆菌,研究微生物菌剂对烟草幼苗农艺性状、根系活力、抗氧化酶相关指标以及烤后烟叶化学成分的影响。结果表明,使用微生物菌剂对烤烟农艺性状、根系活力、抗氧化酶相关指标和烤后烟叶化学成分均有影响。随着烟苗的生长,3种微生物菌剂处理中,添加侧孢芽孢杆菌较对照显著提高了烟苗的根鲜重、根长度、根表面积、根体积及SOD、POD和CAT活性,显著增强了根系活力,增加了可溶性蛋白含量,降低了MDA含量,烤后烟叶化学成分更加协调。因此,在实际生产中,推荐在烟草漂浮育苗营养液中添加侧胞芽孢杆菌促进烟苗根系发育,培育壮苗,提升烟叶品质。

In order to further understand the effects of microbial agents on the growth and development of root in tobacco seedling period,3 microbial agents including Brevibacillus laterosporus,Bacillus subtilis and Bacillus licheniformis were added into the floating seedling nutrient solution.Agronomic traits,root activity,antioxidant enzymes and chemical composition of tobacco leaves after transplantation were determined.The results showed that application of microbial agents had significant effects on agronomic traits,root activity,antioxidant enzymes and chemical components of flue-cured tobacco.Compared with CK,Brevibacillus laterosporus increased the root fresh weight,root length,root surface area and volume of tobacco seedling,improved the activities of SOD,POD and CAT,significantly enhanced the root activity,increased the soluble protein content,reduced the MDA content,and made the leaf tobacco chemical composition more harmonious.So,Brevibacillus laterosporus was recommended as adding microbial agents into the nutrient solution of floating tobacco seedlings,which should promote the root development of tobacco seedlings.The application of Brevibacillus laterosporus could significantly enhance the activity of protective enzymes in the roots of tobacco seedlings,thus improve the growth and development of tobacco roots,cultivate strong seedlings and improve the quality of tobacco leaves.