细胞因子IL-17A对成牙骨质细胞增殖、分化及矿化影响的实验研究

Effects of IL-17A on proliferation,differentiation and mineralization of osteoblasts

目的 探讨IL-17A对成牙骨质细胞增殖、分化及矿化的影响。方法 为研究IL-17A在正畸力致牙根吸收过程中的作用提供理论依据,将OCCM-30细胞置于细胞培养箱内培养,将其分为6组:实验组5组和对照组1组。实验组分别用含10 ng/mL、20 ng/mL、30 ng/mL、40 ng/mL、50 ng/mL IL-17A的培养液处理细胞,对照组使用不含IL-17A的完全培养液,进行如下检测:应用噻唑蓝法(MTT)检测细胞增殖,酶联免疫吸附试验(Elisa)定量检测细胞内碱性磷酸酶活性,茜素红染色法定量测定矿化结节含量,对其结果采用单因素方差分析进行统计学分析。结果 IL-17A作用48 h和72 h后,OCCM-30吸光度值呈浓度依赖性降低,差异有明显统计学意义;在48 h和72 h的时间内,IL-17A对OCCM-30具有促进其ALP活性的作用。不同浓度IL-17A作用于OCCM-30形成矿化结节呈现浓度依赖性降低。结论 Thl7相关细胞因子IL-17A可抑制成牙骨质细胞OCCM-30的增殖、分化及矿化作用。

Objective The purpose of this study was to investigate the effect of IL-17A on the proliferation,differentiation and mineralization of osteoblasts,and to provide a theoretical basis for the study of the role of this cytokine in orthodontic root absorption.Methods OCCM-30 cells were cultured in cell incubator and divided into 6 groups:experimental group 5 and control group The experimental group treated the cells with culture medium containing 10 ng/mL、20 ng/mL、30 ng/mL、40 ng/mL、50 ng/mL IL-17A respectively,and the control group used complete culture medium without IL-17A to carry out the following tests:1.thiazole blue method(MTT)was used to detect cell proliferation,enzyme linked immunosorbent assay(Elisa)was used to quantitatively detect alkaline phosphatase activity in cells,and alizarin red staining was used to determine the content of mineralized nodules.The results were statistically analyzed by one-way ANOVA analysis.Results The OCCM-30 absorbance value decreased in concentration dependence after 48 h and 72 h,and the difference was statistically significant.In 48 h and 72 h,IL-17A OCCM-30 could promote its ALP activity.The concentration of mineralized nodules by different concentrations IL-17A OCCM-30 showed a decrease in concentration dependence.Conclusion Thl7 related cytokine IL-17A can inhibit the proliferation,differentiation and mineralization ofoblast OCCM-30.