孕期慢性缺氧对子代大鼠心脏损伤及氧化应激水平的影响

Impact of Chronic Intrauterine Hypoxia on Cardiac Structure and Oxidative Stress of Offspring Rats

目的研究宫内慢性缺氧造成子代宫内生长受限及不同发育阶段心脏结构变化及其与氧化应激(Oxidative Stress,OS)的关系。方法将怀孕的SD大鼠在妊娠第4~21d(GD4~21)进行缺氧处理,使其子代处于缺氧和缺血状态。对照组为正常饮食的健康大鼠。分别于出生后的第5、20、40d分别测量子代鼠的体重、心脏重量等,同时检查心肌细胞中氧化应激参数丙二醛(malondialdehyde,MDA)含量,抗氧化能力指标谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性和超氧化物歧化酶(Superoxide dismutase,SOD)活力。结果与对照组相比,孕期缺氧诱导后代宫内生长受限,心脏质量指数增高,心肌中MDA生成增多而GSH-Px和SOD的活力下降。结论研究结果表明宫内慢性缺氧与后代心肌结构改变及氧化应激损伤之间存在联系。

Objective To investigate the effects of the maternal hypoxia during pregnancy on the fetal growth,changes of cardiac structure and oxidative stress injury at different developmental stages in offsprings.Methods Pregnant SD rats were treated with chronic intrauterine hypoxia on the 4th to 21st day of gestation(GD4-21)to make their progeny in a hypoxic and ischemic state.The control group was healthy rats fed with normoxic diet.At the 5th,20th and 40th day after birth,the weight and heart weight of the offspring were measured.Cardiac oxidative stress parameters such as MDA,the activity of SOD and GSH-Px were detected.Results Compared with the control group,the Ratio of heart weight to body weight were increased in female offspring submitted to intrauterine hypoxia(HI)during pregnancy.Compared with the control group,HI group shows that MDA was increased but the activity of SOD and GSH-px in the myocardium was decreased that suggesting the heart muscle suffers from oxidative stress injury.Conclusion Our results suggest there was an association among hypoxia during pregnancy and adult vascular structural changes and oxidative stress.