可诱导T细胞共刺激分子在结肠癌组织中的表达情况及其与远期生存的相关性研究

Expression of Inducible T Cell Costimulatory Molecules in Colon Cancer Tissues and Their Correlation with Long-term Survival

目的探讨分析可诱导T细胞共刺激分子(inducible T-cell co-stimulator,ICOS)在结肠癌组织中的表达情况及其与远期生存的相关性。方法选取2018年5月至2019年5月期间在大连大学附属新华医院进行手术切除结肠组织的206例结肠癌患者作为研究对象,取其结肠癌组织及癌旁组织标本,使用荧光定量PCR检测结肠癌组织及癌旁组织中可诱导T细胞共刺激分子表达水平,分析其表达与临床病理特征的关系及远期生存的相关性。结果结肠癌组织中ICOS表达水平(8.73±2.25)显著低于癌旁组织的(18.54±3.26),差异有统计学意义(P<0.05);结肠癌组织中ICOS表达水平与肿瘤直径、远处转移、临床分期、淋巴结转移有关(P<0.05),与年龄、性别、肿瘤部位、分化程度差异无统计学意义(P>0.05);ICOS表达在206例结肠癌组织的中低表达117例,高表达89例;ICOS高表达和低表达与临床病理因素进行Logistic二次回归分析结果显示,ICOS表达与肿瘤大小、临床分期、淋巴结转移、远处转移有关(P<0.05),与年龄、性别、肿瘤位置、分化程度无关(P>0.05);随访截止至2021年9月30日,206例结肠癌患者存活率83.01%(171/206),死亡率16.99%(35/206),中位生存时间为(23.5±3.3)个月。ICOS高表达的89例结肠癌患者的中位生存期为(27.4±3.2)个月,95%CI为2.234~6.147;ICOS低表达的117例结肠癌患者的中位生存期为(16.3±4.3)个月,95%CI为1.458~5.237,ICOS高表达结肠癌患者中位生存期显著高于ICOS低表达者(P<0.05)。结论ICOS在结肠癌组织中呈较低表达,其表达水平与患者远期生存呈正相关关系,其水平可作为结肠癌患者预后生存的重要评估指标。

Objective To explore the expression of inducible T cell costimulatory molecules in colon cancer tissues and their correlation with long-term survival of colon cancer patients.Methods A total of 206 colon cancer patients who underwent surgical resection of colon tissue in Xinhua Hospital Affiliated to Dalian University from May 2018 to May 2019 were selected.Their colon cancer tissues and paracancerous tissues were used for the detection of inducible T-cell co-stimulator(ICOS)expression by fluorescence quantitative PCR.The relationships between the expression level of ICOS and the clinicopathological characteristics or the long-term survival were analyzed.Results The expression level of ICOS was significantly lower in colon cancer tissues(8.73±2.25)than that in adjacent tissues(18.54±3.26)(P<0.05).The expression level of ICOS in colon cancer tissues was related to tumor diameter,distant metastasis,clinical stage,lymph node metastasis(P<0.05),and no relation was found between ICOS expression level and age,gender,tumor location,and degree of differentiation(P>0.05).The expression level of ICOS in 206 colon cancer tissues was low in 117 cases,and high in 89 cases.Logistic regression analysis showed that ICOS expression was related to tumor size,clinical stage,lymph node metastasis,and distant metastasis(P<0.05),but not related to age,gender,lymph node metastasis,tumor location,and degree of differentiation(P>0.05).The 206 colon cancer patients were followed up until September 30,2021,the survival rate was 83.01%(171/206),the mortality rate was 16.99%(35/206),and the median survival time was(23.5±3.3)months.The median survival time of the 89 colon cancer patients with high expression level of ICOS was(27.4±3.2)months,with a 95%CI of 2.24-6.147.The median survival time of the 117 colon cancer patients with low expression level of ICOS was(16.3±4.3)months,and the 95%CI was 1.458-5.237.The median survival time of colon cancer patients with high expression level of ICOS was significantly higher than that of patients with low expression level of ICOS(P<0.05).Conclusion ICOS is lowly expressed in colon cancer tissues,and its expression level is positively correlated with the long-term survival of patients.The expression level of ICOS can be used as an important evaluation index for the prognosis and survival of colon cancer patients.