载脂蛋白E基因多态性与妊娠期高血压疾病的关系

Relationship between ApoE gene polymorphism and hypertensive of disorders pregnancy

目的分析载脂蛋白E(ApoE)基因多态性与妊娠期高血压疾病(HDP)的关系。方法选取2016年1月至2020年12月在广东省佛山市第一人民医院就诊及分娩的327例产妇为研究对象,其中162例HDP患者为病例组,165例健康妊娠妇女为对照组,采用基因芯片法检测ApoE基因多态性,分析两组对象间ApoE基因型及基因频率差异,并比较不同ApoE基因型人群的血脂水平差异。结果两组临床特征比较,除了高密度脂蛋白胆固醇在对照组较高外,病例组的血压、总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-c)水平及蛋白尿、水肿、胎儿生长受限、羊水过少的发生率均高于对照组(P<0.05)。两组的ApoE基因型均以ε3/3基因型为主,病例组的ε2/3、ε3/3基因型频率均显著低于对照组(4.94%vs.13.94%,P<0.05;59.88%vs.71.52%,P<0.05),ε3/4、ε4/4基因型频率均显著高于对照组(22.22%vs.6.67%,P<0.05;8.02%vs.1.82%,P<0.05)。ε3等位基因是主要等位基因,ε2、ε3等位基因在病例组的频率低于对照组(P<0.05),但ε4等位基因高于对照组(20.68%vs.6.36%,P<0.05)。E4表型人群的TC、TG及LDL-c水平均显著高于E2和E3表型人群(P<0.05)。结论ApoEε4等位基因与妊娠期血脂代谢异常及HDP的发生有关,而携带ε2等位基因的人群患HDP的概率较低。

Objective To analyze the relationship between apolipoprotein E(ApoE)gene polymorphism and hypertensive of disorders pregnancy(HDP).Methods The clinical datas of 327 parturients who attended and gave birth in the First People′s Hospital of Foshan from January 2016 to December 2020 were collected for a case-control study.162 patients with HDP were divided into the case group,and 165 healthy pregnant women were divided into the control group.The gene chip method was used to detect ApoE gene polymorphism of the subjects.The ApoE genotype and gene frequency difference between the two groups were analyzed.The serum lipid levels of different ApoE genotypes were also compared.Results The comparison results of the clinical characteristics of the two groups showed that in addition to the higher high density lipoprotein cholesterol(HDL-c)valued in the control group,other indicators in the case group[blood pressure,total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterin(LDL-c)and the incidence of proteinuria,edema,fetal growth restriction,oligohydramnios]were significantly higher than those in the control group(P<0.05).ε3/3 was the main genotype in the two groups.Theε2/3 andε3/3 genotype frequencies in the case group were significantly lower than those in the control group(4.94%vs.13.94%,P<0.05;59.88%vs.71.52%,P<0.05),but the genotype frequencies ofε3/4 andε4/4 were significantly higher than those in the control group(22.22%vs.6.67%,P<0.05;8.02%vs.1.82%,P<0.05).Theε3 allele was the main allele.The frequency of theε3 allele in the case group was lower than that in the control group(P<0.05),but theε4 allele was higher than that in the control group(20/68%vs.6.36%,P<0.05).The levels of TC,TG and LDL-c of the E4 population were significantly higher than those of the E2 and E3 population(P<0.05).Conclusion ApoEε4 is closely related to dyslipidemia and HDP,the population with ApoEε2 may have a lower probability of HDP.