75%摄食量对尿酸酶缺失大鼠的降尿酸作用

Serum Uric Acid Lowered by 75% Dietary Intake in Uricase-deficient Rats

为观察不同摄食量对尿酸酶缺失大鼠(昆明DY大鼠)血尿酸影响,将45 d日龄雄性昆明DY大鼠随机分成禁食组(禁食2d)或节食组(即给予正常摄食量的90%、80%、75%、70%、60%、50%、25%,持续14 d);实验前、中、后断尾取血制备血清,记录体质量、24 h摄食和饮水量,收集大鼠24 h尿液和粪便,并使用磷钨酸法检测样品尿酸含量。结果表明,禁食2 d会使大鼠血尿酸会显著升高,每日给予大鼠正常摄食量的75%,其血尿酸、肌酐、总胆固醇和甘油三酯14 d后呈显著下降趋势,且体质量维持稳定;其余不同摄食量的组分对大鼠血尿酸有不同程度的增加倾向或无明显影响。综上,摄食量控制在75%,对尿酸酶缺失大鼠具有降尿酸的作用,同时对肾功和血脂也有一定的改善作用。

In order to observe the effect of food intake on serum uric acid(SUA), male uricase-deficient rats(Kunming-DY rats) aging 45 days were randomly divided into fasting group(fasting for 2 days) or dieting groups(90%, 80%,75%, 70%, 60%, 50% and 25% of normal food intake for 14 days). Before, during and after the experiment, blood samples were drawn from the tail to prepare serum. In the same time, body weight, 24-hour food intake and water consumption were recorded, and 24-hour urine and feces of rats were collected. The uric acid in samples was detected by uric acid assay kits of phosphotungstic acid method. The results showed that SUA in fasting group significantly increase. SUA in the rats that received 75% of the normal food supply was lowered. Besides, creatinine, total cholesterol and triglyceride in their serum decreased significantly after 2-weeks’ treatment, with their stable body weight. Other food intakes had almost no significant effect on, or increase SUA in Kunming-DY rats. In conclusion, 75% food intake lower SUA in uricase-deficient rats with their improvement of renal function and blood lipid.