驴油提升皮肤屏障功能及潜在作用机制研究

The effect of donkey oil on the improvement of skin barrier function and its potential mechanism

探讨驴油提升皮肤屏障功能的作用及其可能的作用机制。将十二烷基硫酸钠作用于体外重组三维表皮模型(EpiKutis)构建皮肤屏障损伤模型,采用四唑盐比色法(MTT)检测组织活力,利用酶联免疫吸附检测方法(ELISA)检测IL-1α的水平,考察驴油对人3D皮肤屏障损伤模型的屏障指数和IL-1α水平的影响。采用转录组高通量测序(RNAseq)技术揭示驴油对皮肤生理的机制,并揭示驴油提升皮肤屏障功能的可能机制。结果表明,驴油可显著提升人3D皮肤屏障损伤模型的屏障指数和降低IL-1α水平,效果与地塞米松类似。驴油作用于人3D皮肤模型后,筛选出1649个差异表达基因,其中619个基因上调,1030个基因下调。基因本体论(Gene Ontology,GO)功能富集分析表明差异表达基因与表皮发育、表皮细胞分化及皮肤发育有关;京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析结果表明差异基因参与TGF-β信号通路、IL-17信号通路、HIF-1信号通路、细胞因子-细胞因子受体相互作用、NF-κB信号通路等重要生物学通路,其中与驴油提升皮肤屏障功能有关的通路可能是TGF-β信号通路、HIF-1信号通路和NF-κB信号通路。

The aim of the study was to explore the effect of donkey oil in enhancing the skin barrier function and its possible mechanism.Sodium lauryl sulfate was applied to the three-dimensional epidermal model(EpiKutis)to construct the skin barrier damage model,the tissue activity was detected by the MTT method and the level of IL-1αby the ELISA method.It was used to study the effect of the donkey oil on the barrier index and IL-1αlevel of the SLS-induced EpiKutis.RNA-seq technology was used to explain the physiological mechanism of donkey oil on the skin,and to reveal the possible mechanism of donkey oil to enhance the skin barrier function.The results show that donkey oil can significantly increase the barrier index of the SLS-EpiKutis model and reduce the level of IL-1α,and the effect is similar to that of dexamethasone.After the donkey oil acting on the EpiKutis model,1649 differentially expressed genes are screened,of which 619 genes are up-regulated and 1030 genes are down-regulated.GO functional enrichment analysis shows that differentially expressed genes are related to epidermal development,epidermal cell differentiation and skin development.KEGG pathway enrichment analysis results show that differential genes are involved in TGF-βsignaling pathway,IL-17 signaling pathway,HIF-1 signaling pathway,and cell factor-cytokine receptor interaction,NF-κB signaling pathway and other important biological pathways.The pathways related to donkey oil enhancing the skin barrier function may be TGF-βsignaling pathway,HIF-1 signaling pathway and NF-κB signaling pathway.