烟酰胺与茶多酚复配对抑制PIG1细胞黑色素的影响

Effects of nicotinamide mixed with tea polyphenols on inhibiting melanin in PIG1 cells

研究了烟酰胺及其与茶多酚复配组分对黑色素生物合成及转运机制的作用,以促黑素细胞激素(α-MSH)诱导黑色素高表达的正常人皮肤黑色素细胞(PIG1细胞)为研究模型,通过NaOH裂解比色法测定PIG1细胞黑色素含量,多巴氧化比色法测定PIG1细胞内酪氨酸酶活性,免疫印迹法(Western blot)检测PIG1细胞内调节黑色素生物合成及转运关键受体酪氨酸酶相关蛋白1(TRP-1)、酪氨酸酶相关蛋白2(TRP-2)、黑皮质素受体1(MC1R)及Rab27a蛋白表达情况。结果表明,当2 g/L烟酰胺+0.5 g/L茶多酚、3 g/L烟酰胺+0.3 g/L茶多酚、3 g/L烟酰胺+0.5 g/L茶多酚复配组合时,对α-MSH诱导黑色素高表达的PIG1细胞中黑色素含量、细胞内酪氨酸酶活性、TRP-1、TRP-2、MC1R及Rab27a蛋白均存在极显著的抑制作用,并强于5 g/L烟酰胺,而当烟酰胺质量浓度为2 g/L时仅对细胞内酪氨酸酶活性存在抑制作用,即复配组合具有更显著的抑制PIG1细胞中黑色素生物合成及转移的作用。

The aim was to study and compare the effects of nicotinamide and its complexes with tea polyphenols on melanin biosynthesis and transport mechanism.Taking human skin melanocytes PIG1 cell line with high melanin expression induced by melanocyte-stimulating hormone(α-MSH)as the model,the effects of nicotinamide and its complexes matching with tea polyphenols on melanin content,intracellular tyrosinase activity and the key receptors in melanin biosynthesis and transport pathway were determined via western blot and colorimetric method with NaOH and L-DOPA.The results show that 2 g/L nicotinamide+0.5 g/L tea polyphenols,3 g/L nicotinamide+0.3 mg/L tea polyphenols,3 g/L nicotinamide+0.5 g/L tea polyphenols complexes show significantly inhibitory effects on melanin content,intracellular tyrosinase activity,the expression of tyrosinase-related protein-1(TRP-1),tyrosinase-related protein-2(TRP-2),melanocyte melanin cortisol receptor(MC1R)and human epidermal melanocyte transport-related proteins(Rab27a)protein inα-MSH-induced high melanin expression human PIG1 cell lines,which are stronger than that of 5 g/L nicotinamide.While 2 g/L nicotinamide only has the effects of suppressing intracellular tyrosinase activity.The combination of nicotinamide and tea polyphenols had more obvious effect to suppress the melanin biosynthesis and transport pathway in PIG1 cell lines.