高效液相色谱-串联质谱法快速测定护肤透明质酸中抗菌药物

Rapid determination of antibacterial agents in skin care hyaluronic acid by high performance liquid chromatography-tandem mass spectrometry

建立护肤透明质酸中抗生素类和硝基咪唑类两大类抗菌药物的高效液相色谱-串联质谱快速测定方法。分别选取两类抗菌药物各10种试剂,通过涡旋振荡、超声提取以及离心处理和过滤后,制备了两类抗菌药物的混合溶液。经色谱柱分离,梯度洗脱下通过正离子模式电喷雾电离,多反应监测模式检测。测定结果表明:两种抗菌药物的溶液样本中,各自含有的10种化合物成分的总离子均在不同时间下发生分离,并产生相应的响应值,呈现较好的线性关联,相关系数分别在0.997和0.996以上。两种抗菌药物的检出限、定量限分别为1.6,6 mg/kg和0.9~201,5~401 mg/kg。该方法准确、高效,适用于护肤透明质酸中抗生素类和硝基咪唑类抗菌药物的快速测定。

The aim was to establish a rapid method for the determination of antibiotics and nitroimidazoles in skin care hyaluronic acid by high performance liquid chromatography tandem mass spectrometry.Ten reagents for each of the two kinds of antibiotics were selected.After vortex oscillation,ultrasonic extraction,centrifugation and filtration,the mixed solution samples of the two kinds of antibiotics were prepared.They were separated by chromatographic column,ionized by electrospray ionization in positive ion mode and detected by multiple reaction monitoring mode under gradient elution.The results show that the total ions of 10 compounds contained in the solution samples of the two antibiotics have separation reactions at different times and produce corresponding response values,showing a good linear correlation.The correlation coefficient results are 0.997 and above 0.996,respectively.The detection limits and quantitative limits of the two antibiotics are 1.6,6 mg/kg,0.9-201 mg/kg and 5-401 mg/kg,respectively.The method can accurately and efficiently complete the rapid determination of antibiotics and nitroimidazoles in skin care hyaluronic acid.