银杏内酯B对人结肠癌SW480细胞恶性生物学行为及ROS/XIAP信号通路的影响

Effect of Ginkgolide B on the Malignant Biological Behavior of Human Colorectal Cancer SW480 Cells and ROS/XIAP Signaling Pathway

目的探讨银杏内酯B对人结肠癌SW480细胞恶性生物学行为及活性氧(ROS)/X连锁凋亡蛋白抑制剂(XIAP)信号通路的影响。方法实验分为模型组(培养基+SW480细胞)、紫杉醇组(5 mg/L+培养基+SW480细胞),以及银杏内酯B低、高剂量组(50,100μmol/L+培养基+SW480细胞)。采用四唑盐(MTT)法测定细胞增殖水平并计算存活率,流式细胞术测定细胞凋亡情况并计算凋亡率,Transwell法测定细胞迁移侵袭水平,荧光探针法检测活性氧(ROS)水平,反转录聚合酶链反应法及Westernblot法测定细胞XIAPmRNA及蛋白表达水平。结果与模型组比较,紫杉醇组及银杏内酯B低、高剂量组细胞存活率显著降低,凋亡率显著升高,细胞侵袭水平显著降低,细胞ROS水平及XIAPmRNA和蛋白表达水平均显著降低(P<0.05)。结论银杏内酯B可有效抑制SW480细胞的迁移和侵袭并诱导其凋亡,其机制可能与抑制ROS/XIAP信号通路的激活有关。

Objective To investigate the effect of ginkgolide B on the malignant biological behavior of human colorectal cancer SW480 cells and reactive oxygen species(ROS)/X-linked apoptosis protein inhibitor(XIAP)signaling pathway.Methods The model group(medium+SW480 cells),paclitaxel group(5 mg/L of paclitaxel+medium+SW480 cells)and ginkgolide B low-and high-dose groups(50,100μmol/L of ginkgolide B+medium+SW480 cells)were set up in the experiment.Methyl thiazolyl tetrazolium(MTT)assay was used to measure the level of cell proliferation and calculate the survival rate,flow cytometry was used to measure the rate of cell apoptosis,Transwell method was used to measure the level of cell migration and invasion,fluorescent probe method was used to measure the level of reactive oxygen species(ROS),reverse transcription-polymerase chain reaction and Western blot were used to measure the expression level of XIAP mRNA and protein.Results Compared with those in the model group,the survival rate of SW480 cells was significantly lower,the apoptosis rate of SW480 cells was significantly higher,while the invasion level,ROS level and expression level of XIAP mRNA and protein of SW480 cells were significantly lower in the paclitaxel group and ginkgolide B low-and high-dose groups(P<0.05).Conclusion Ginkgolide B can effectively inhibit the migration,invasion and apoptosis of SW480 cells,and its mechanism may be related to the inhibition of the activation of ROS/XIAP signaling pathway.