核黄素单线态氧光化学法的应用及检测研究进展

Progress in application and detection of riboflavin singlet oxygen photochemistry

光动力疗法(PDT)主要机制是利用光敏剂产生单线态氧杀死细胞,早在100多年前就被科学家们所发现,目前已经广泛被用于临床治疗。核黄素是1种内源性光敏剂,是最常用的光敏剂,大量研究实验发现核黄素光照后可通过Ⅱ型光动力反应产生单线态氧,该方法目前广泛用于杀灭血液中细菌、病毒等病原体,而单线态氧被认为是光动力效应中的关键因子。单线态氧可诱导细胞凋亡、细胞坏死及细胞自噬。目前检测单线态氧的方法有直接检测法和间接检测法,但都还存在一定的局限性。本文就光动力法作用于核黄素产生单线态氧的产生机制、作用机制及单线态氧的检测进行综述,为进一步将光动力疗法应用于临床以及寻找单线态氧的更合适检测方法提供依据。

Photodynamic therapy(PDT),which uses photosensitizers to produce singlet oxygen to kill bacteria, was first discovered by scientists over 100 years ago and has been widely used in clinical treatment. Riboflavin is an endogenous and the most commonly used photosensitizer.A large number of studies have shown that riboflavin can produce singlet oxygen through photodynamic reaction(typeⅡ) after illumination.This method is currently widely used to kill pathogens such as bacteria and viruses in blood, and singlet oxygen is considered to be the key factor in the photodynamic effect.Singlet oxygen can induce cell apoptosis, necrosis and autophagy.At present, there are direct and indirect methods to detect singlet oxygen, but both with some limitations.This paper reviews production mechanism, action mechanism and detection of singlet oxygen produced by riboflavin during photodynamic therapy, which provides a basis for further application of photodynamic therapy in clinical practice and searching for a more suitable detection method for singlet oxygen.