LncRNA SNHG14调控miR-142-5p对寡聚态Aβ诱导的鼠源神经细胞系PC12损伤的影响

Effect of lncRNA SNHG14 on oligomeric Aβ-inducedinjury in rat neural cell line PC12 through regulating miR-142-5p

目的探讨lncRNA SNHG14靶向miR-142-5p对寡聚态Aβ诱导的鼠源神经细胞系PC12损伤的影响。方法将PC12细胞分为对照组、模型组、模型+si-NC组、模型+si-SNHG14组、模型+miR-NC组、模型+miR-142-5p组、模型+si-SNHG14+anti-miR-NC组、模型+si-SNHG14+anti-miR-142-5p组。RT-qPCR检测SNHG14和miR-142-5p水平。CCK-8和流式细胞术测量细胞增殖抑制率和凋亡率。ELISA检测培养液中TNF-α、IL-6和IL-10水平。双荧素酶报告实验确定SNHG14和miR-142-5p的靶向关系。结果AD患者血浆中SNHG14呈高表达(P<0.05),miR-142-5p呈低表达(P<0.05)。与对照组比较,模型组细胞SNHG14水平、增殖抑制率、凋亡率显著升高(P<0.05),miR-142-5p水平显著降低(P<0.05),培养液中TNF-α和IL-6水平显著升高(P<0.05),IL-10水平显著降低(P<0.05)。与model+si-NC组比较,model+si-SNHG14组细胞增殖抑制率、凋亡率显著降低(P<0.05),培养液中TNF-α和IL-6水平显著降低(P<0.05),IL-10水平显著升高(P<0.05)。与model+miR-NC组比较,model+miR-142-5p组细胞增殖抑制率、凋亡率显著降低(P<0.05),培养液中TNF-α和IL-6水平显著降低(P<0.05),IL-10水平显著升高(P<0.05)。miR-142-5p是SNHG14的直接靶点。与model+si-SNHG14+anti-miR-NC组比较,model+si-SNHG14+anti-miR-142-5p组细胞增殖抑制率、凋亡率显著升高(P<0.05),培养液中TNF-α和IL-6水平显著升高(P<0.05),IL-10水平显著降低(P<0.05)。结论沉默SNHG14通过靶向促进miR-142-5p表达能够减轻寡聚态Aβ诱导的神经细胞损伤。

Objective To investigate the effect of lncRNA SNHG14 targeting at miR-142-5p on the neuronal damage induced by oligomeric Aβ.Methods PC12 cells were divided into control group,model group,model+si-NC group,model+si-SNHG14 group,model+miR-NC group,model+miR-142-5p group,model+si-SNHG14+anti-miR-NC group,and model+si-SNHG14+anti-miR-142-5p group.The level of SNHG14 and miR-142-5p wascalculated by RT-qPCR.CCK-8 method and flow cytometry were applied to detect inhibition rate on cell proliferation and apoptosis rate.ELISA method was used to detect TNF-αand IL-6 in the culture.Dual luciferase report assay was used to find the targeting relationship between SNHG14 and miR-142-5p.Results SNHG14 was highly expressed(P<0.05)while miR-142-5p was lowly expressed(P<0.05)in plasma of AD patients.Compared with the control group,the SNHG14 level,cell proliferation inhibition rate,and apoptosis rate in the model group were significantly increased(P<0.05),the level of miR-142-5p was significantly reduced(P<0.05),and the levels of TNF-αand IL-6 in the culture medium were significantly increased(P<0.05),IL-10 level was significantly decreased(P<0.05).Compared with the model+si-NC group,the cell proliferation inhibition rate and apoptosis rate in the model+si-SNHG14 group were significantly reduced(P<0.05),the level of TNF-αand IL-6 in the culture medium was significantly reduced(P<0.05),IL-10 level was significantly increased(P<0.05).Compared with the model+miR-NC group,the cell proliferation inhibition rate and apoptosis rate in the model+miR-142-5p group were significantly reduced(P<0.05),and the levels of TNF-αand IL-6 in the culture medium were significantly reduced(P<0.05)and IL-10 level was significantly increased(P<0.05).miR-142-5p was a direct target of SNHG14.Compared with the model+si-SNHG14+anti-miR-NC group,the cell proliferation inhibition rate and apoptosis rate in the model+si-SNHG14+anti-miR-142-5p group were significantly increased(P<0.05),and the levels of TNF-αand IL-6 in the culture medium were significantly increased(P<0.05),IL-10 level was significantly decreased(P<0.05).Conclusions Silencing SNHG14 may alleviate oligomeric Aβ-induced neuronal damage by targeting at and promoting miR-142-5p expression.