益气化瘀方调控PI3K/AKT/mTOR信号通路对糖尿病溃疡大鼠外周神经修复的作用

Effect of Yiqi Huayu Decoction on Nerve Repair in Rats with Diabetic Ulcer

目的:探讨益气化瘀方调控PI3K/AKT/mTOR信号通路对糖尿病溃疡大鼠外周神经修复的作用。方法:选取健康雄性SPF级SD大鼠30只,分成正常组6只,其余24只大鼠建立糖尿病周围神经病变溃疡模型,然后随机分为模型组、益气化瘀方组、益气化瘀方+PI3K抑制剂组、益气化瘀方+mTOR抑制剂组,每组6只。给药第21天后处死动物取材,观察大鼠坐骨神经形态,检测神经传导速度、创面神经肽P物质、神经生长因子(NGF)蛋白及NGF mRNA表达水平。结果:与正常组比较,模型组坐骨神经病变程度更重,神经传导速度减慢,P物质、NGF蛋白及NGF mRNA含量减少,差异有统计学意义(P<0.05);与模型组比较,益气化瘀方组坐骨神经病变程度减轻,神经传导速度加快,P物质、NGF蛋白及NGF mRNA含量增加,差异有统计学意义(P<0.05);与益气化瘀方组比较,益气化瘀方+PI3K抑制剂组、益气化瘀方+mTOR抑制剂组坐骨神经病变程度更重,神经传导速度减慢,P物质、NGF蛋白及NGF mRNA含量减少,差异有统计学意义(P<0.05)。结论:益气化瘀方可能通过调控PI3K/AKT/mTOR信号通路影响P物质、NFG表达,促进糖尿病溃疡大鼠外周神经的修复。

Objective To investigate the effect of Yiqi Huayu Decoction in regulating PI3K/AKT/mTOR signal pathway on peripheral nerve repair in rats with diabetic ulcer.Methods Thirty healthy male(SPF grade)SD rats were established a diabetic peripheral neuropathy ulcer model,then they were randomly divided into model group,Yiqi Huayu Decoction group,Yiqi Huayu Decoction+PI3K inhibitor group,Yiqi Huayu Decoction+mTOR inhibitor group.The sciatic nerve morphology of rats on the 21st day after administration was observed and the nerve conduction velocity,wound neuropeptide P substance(SP),NGF protein and NGF mRNA content were detected.Results Compared with the control group,the sciatic neuropathy of the model group was more severe.The nerve conduction speed was slowed down,and the contents of SP,NGF protein and NGF mRNA were decreased,which the difference were statistically significant(P<0.05).Compared with the model group,the sciatic neuropathy of the Yiqi Huayu Decoction group reduced,nerve conduction speed increased,SP,NGF protein and NGF mRNA content increased(P<0.05).Compared with Yiqi Huayu Decoction group,the degree of sciatic neuropathy in the Yiqi Huayu Decoction+PI3K inhibitor group and Yiqi Huayu Decoction+mTOR inhibition group was more serious,nerve conduction speed was slowed down,and the contents of SP,NGF protein and NGF mRNA were decreased,which the difference were statistically significant(P<0.05).Conclusion Yiqi Huayu Decoction may improve the expression of SP and NFG by regulating the PI3K/AKT/mTOR signal pathway,and promote the repair of peripheral nerves in diabetic ulcer rats.