楚雄地区黄龙尾(Agrimonia pilosa var.nepalensis)内生菌的分离及初步鉴定

Isolation and Preliminary Identification of Endophytes of Agrimonia pilosa var. nepalensis in Chuxiong

从野生黄龙尾健康植株内分离得到的29株内生菌中,8株细菌全部为杆菌,有4株产芽孢;10株放线菌,都属于链霉菌属(Streptomyces sp.);11株真菌,其中7株为镰刀菌属(Fusarium sp.)、2株为炭疽菌属(Colletotrichum sp.)、1株为青霉属(Penicillium sp.)、1株为黑曲霉(Aspergillus niger)。生长特性实验结果表明,所有供试菌株的最适生长NaCl浓度范围在3%-5%之间,最适生长pH范围在6.0~8.0之间,最适生长温度在20℃~35℃之间,细菌的生长温度范围比放线菌的更为广泛。在生理生化特性实验中,供试菌株B-3-5-4对碳源、氮源的利用范围广,菌株B-3-1-6对碳源、氮源的利用范围窄。研究结果表明,黄龙尾不同组织内部分布着不同类群的内生菌群。本研究可为黄龙尾的进一步开发提供数据支持。

29 endophytes were isolated from healthy plants of wild Agrimonia pilosa var.nepalensis,in‐cluding 8 strains of bacteria,10 strains of actinomycetes and 11 strains of fungi.All the bacteria were bacilli and 4 strains produced spores.All actinomycetes belong to Streptomyces sp..Fungi belong to 4 genera,7strains are Fusarium sp.,2 strains are Colletotrichum sp.,1 strain is Penicillium sp.,1 strain is Aspergillus ni‐ger.The results of growth characteristics experiment showed that the optimal growth range of all tested strains was 3%~5%NaCl concentration,6.0~8.0 pH and 20℃~35℃.The growth temperature range of bacteria was wider than that of actinomycetes.In the experiment of physiological and biochemical characteristics,strain b-3-5-4 used a wide range of carbon and nitrogen sources,while strain b-3-1-6 used a narrow range of carbon and nitrogen sources.The results showed that there were different groups of endophytes in different tis‐sues of Agrimonia pilosa var.nepalensis.This study can provide data for further development of Agrimonia pi‐losa var.nepalensis.