冷压初榨椰子油对四氯化碳致小鼠急性肝损伤的保护作用

Hepatoprotective effects of virgin cold-pressed coconut oil against carbon tetrachloride induced acute liver injury in mice

研究了冷压初榨椰子油对四氯化碳(CCl;)诱导昆明小鼠急性肝损伤的保护作用。将72只昆明小鼠随机分成6组,包括正常组、四氯化碳肝损伤模型组、联苯双酯组(150 mg/kg)、椰子油低剂量组(5 mL/kg)、椰子油中剂量组(10 mL/kg)、椰子油高剂量组(20 mL/kg),通过测定小鼠血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、乳酸脱氢酶(LDH)、甘油三酯(TG)和总胆固醇(TC)指标,肝组织中超氧化物歧化酶(SOD)活力、谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量,采用HE染色切片对小鼠肝脏组织进行病理学检查,考察冷压初榨椰子油对四氯化碳致小鼠急性肝损伤的保护作用。结果表明:与模型组相比,不同剂量组的冷压初榨椰子油(5、10 mL/kg和20 mL/kg)可以不同程度地降低小鼠血清中ALT、AST、LDH、TG、TC水平以及肝组织中MDA含量,提高肝组织中SOD活力与GSH-Px活力;同时,肝脏组织病理学观察与生化指标检测结果一致。其中,高剂量的冷压初榨椰子油(20 mL/kg)作用效果优于阳性对照联苯双酯。研究表明冷压初榨椰子油对肝脏具有较好的保护作用,作用机制可能与其含有较多的抗氧化物质以及功能性物质有关。

The hepatoprotective effects of virgin cold-pressed coconut oil(VCPCO)against carbon tetrachloride(CCl;)induced acute liver injury in Kunming(KM)mice were studied.A total of 72 Kunming mice were randomly divided into 6 groups,including normal group,CCl;liver injury model group,bifendate group(150 mg/kg),coconut oil low dosage group(5 mL/kg),coconut oil medium dosage group(10 mL/kg)and coconut oil high dosage group(20 mL/kg).The levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),lactate dehydrogenase(LDH),triglyceride(TG),total cholesterol(TC)in serum,and superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)in liver were determined.The liver tissue was examined pathologically by HE staining section.The results showed that VCPCO with dosages of 5,10 mL/kg and 20 mL/kg could decrease the levels of ALT,AST,LDH,TG,TC in serum and MDA in liver,and increase the levels of SOD and GSH-Px in liver.Meanwhile,the results of liver histopathology were consistent with those of biochemical markers.Among them,the effect of high dosage of VCPCO(20 mL/kg)was better than the positive control bifendate.Results indicated that VCPCO had a good protective effect on liver,and the mechanism might be related to its high content of antioxidative and functional compounds.