改良机械损伤法建立大鼠子宫内膜损伤模型

The rat endometrial injury model established by modified mechanical injury method

目的通过改良机械损伤法建立大鼠内膜损伤模型,模拟并分析内膜损伤-修复中的宫腔粘连形成过程,为临床防治宫腔粘连提供实验依据。方法27只雌性SD大鼠,按随机数字法随机分为空白组(n=9)、造模组(n=9)、对照组(n=9)。空白组不进行任何干预操作;造模组利用改良机械损伤法损伤子宫内膜;对照组行假手术。分别检测术后第1、4、7天各组子宫内膜腺体数量、纤维化程度、微血管密度、宫腔粘连因子(TGF-β1、VEGF)的时空表达来进行评估模型。结果造模组较对照组及空白组内膜腺体数目减少(P<0.05),子宫内膜变薄,子宫内膜纤维化面积增加,子宫内膜微血管密度略增多,增加了TGF-β1、VEGF的表达。结论改良机械损伤法建立大鼠内膜损伤模型成立。

Objective The modified mechanical injury method was used to establish the rat endometrial injury model to simulate the endometrial injury and repair process during the formation of intrauterine adhesions.The temporal and spatial changes of endometrium during the repair process were analyzed to study the clinical entry point of preventing intrauterine adhesions.Methods Twenty seven female SD rats were randomly divided into blank group,model group and control group according to Random number method.The blank group(n=9)did not carry out any intervention operation;the model group(n=9)received modified mechanical injury method to damage endometrium;the control group(n=9)underwent sham operation.The number of glands,degree of endometrial fibrosis,microvessel density of rat endometrium,temporal and spatial expression of TGF-β1 and VEGF were examined on the 1st,4th and 7th day after operation.Results After mechanical injury,the number of endometrial glands decreased,the fibrotic area of endometrium increased,and the microvessel density of endometrium was slightly higher,with increased the expression of TGF-β1 and VEGF.Conclusion The rat intimal injury model was established by improved mechanical injury method.