不稳定性心绞痛代谢组学研究

Metabolomics of unstable angina pectoris

目的通过液相色谱-质谱联用技术,探讨不稳定性心绞痛(UAP)患者的代谢分子标志物。方法选取2021年3~9月我中心高压氧科住院的UAP患者11例为UAP组,另选取同期住院的非冠心病患者10例为对照组,通过液相色谱-质谱联用技术,进行代谢组学检测2组差异代谢产物。结果UAP组治疗前收缩压[(131.21±13.40)mm Hg(1 mm Hg=0.133 kPa)vs(116.40±12.41)mm Hg]、空腹血糖[(6.97±2.24)mmol/L vs(4.87±0.25)mmol/L]、TG[(1.93±1.10)mmol/L vs(1.11±0.54)mmol/L]、N末端B型钠尿肽前体(NT-proBNP)水平[(131.20±105.42)ng/L vs(42.52±37.61)ng/L]明显高于对照组(P<0.05)。UAP组治疗后收缩压、舒张压、TC、LDL-C、NT-proBNP水平明显低于治疗前,差异有统计学意义(P<0.05)。UAP组治疗前后匹配到3种差异代谢物:甘氨脱氧胆酸、牛磺脱氧胆酸和油酰胺(P<0.05)。治疗前UAP组与对照组匹配到差异代谢物10种(P<0.05,P<0.01)。治疗前UAP组与治疗后和对照组共同匹配的差异代谢物有甘氨脱氧胆酸和牛磺脱氧胆酸。结论发现3种可作为UAP标志物的代谢产物,分别是甘氨脱氧胆酸、牛磺脱氧胆酸和油酰胺。

Objective To explore the metabolic molecular markers in patients with unstable angina pectoris(UAP)by chromatography-mass spectrometry.Methods A total of 11 UAP patients admitted to our hospital from March 2021 to September 2021 were selected as an UAP group,and another 10 patients without coronary heart disease hospitalized in the same period served as a control group.Their baseline data were collected.Liquid chromatography-mass spectrometry was used to detect the metabolism in 2 groups to screen and analyze the different metabolites.Results Befroe treatment,the UAP group had significantly higer SBP(131.21±13.40 mm Hg vs 116.40±12.41 mm Hg,1 mm Hg=0.133 kPa),and fasting blood glucose(6.97±2.24 mmol/L vs 4.87±0.25 mmol/L),TG(1.93±1.10 mmol/L vs 1.11±0.54 mmol/L)and NT-proBNP levels(131.20±105.42 ng/L vs 42.52±37.61 ng/L)than the control group(P<0.05).The SBP,DBP,TC,LDL-C and NT-proBNP levels after treatment were significantly decreased than those before treatment in the UAP group(P<0.05).There were 3 different metabolites down-regulated in the UAP group after treatment,as glycodeoxycholic acid,taurodeoxycholic acid and oleamide(P<0.05).Before treatment,10 different metabolites were detected between the 2 groups(P<0.05,P<0.01).Overlapping differential metabolites were glycodeoxycholic acid and taurodeoxycholic acid in the UAP group before and after treatment.Conclusion Three metabolites may be used as markers of UAP,and they are glycodeoxycholic acid,taurodeoxycholic acid and oleamide.