酶菌协同发酵产青钱柳多糖工艺优化

Optimization for production process of polysaccharide from Cyclocarya paliurus by co-fermentation with enzyme and microbe

以青钱柳干燥后叶子为原料,青钱柳多糖的含量为评价指标,采用复合酶(纤维素酶与半纤维素酶质量比1∶1)对原料进行酶解处理,再经过混合菌种(粗糙脉孢菌(Neurospora crassa)和枯草芽孢杆菌(Bacillus subtilis)质量比1∶1)对酶解液进行发酵,并采用单因素试验及响应面试验对酶菌协同发酵产青钱柳多糖的工艺进行优化。结果表明,最优工艺条件为:料液比8∶100(mg∶mL)、纤维素酶与半纤维素酶质量比1.8∶1.0、复合酶添加量0.8%、酶解时间2.5 h、蔗糖添加量8.0%、粗糙脉孢菌和枯草芽孢杆菌质量比2∶1、混合菌添加量5.0%、发酵时间60 h。在此优化条件下,青钱柳多糖含量达到3.34 mg/mL。

Using the dried leaves of Cyclocarya paliurus as raw materials,and the contents of polysaccharide in C.paliurus as evaluation index,the material was hydrolyzed with mixed enzymes(cellulase and hemi cellulase mass ratio 1∶1),the enzymolysis solution was fermented by mixed microbes(Neurospora crassa and Bacillus subtilis mass ratio 1∶1),and the process for polysaccharide production of C.paliurus by co-fermentation with enzymes and microbes was optimized by single factor tests and response surface tests.The results showed that the optimal fermentation conditions were as follows:solid and liquid ratio 8∶100(mg∶ml),cellulase and hemi cellulase mass ratio 1.8∶1.0,compound enzyme addition 0.8%,enzymolysis time 2.5 h,sucrose addition 8.0%,N.crassa and B.subtilis mass ratio 2∶1,mixed microbes addition 5.0%,and fermentation time 60 h.Under the optimal conditions,the contents of polysaccharide from C.paliurus reached 3.34 mg/ml.