脉络膜新生血管动物模型的研究进展

Research progress on animal models of choroidal neovascularization

脉络膜新生血管(CNV)是多种眼底疾病共同的病理表现,建立成模率高、重复性好、成本低的CNV动物模型对于深入研究CNV的发病机制、病理改变及临床治疗等具有重要意义。目前常用的CNV动物模型的建模方法有激光诱导、视网膜下注射生长因子诱导及基因诱导等;评价方法主要有眼底荧光血管造影、吲哚菁绿血管造影、苏木精-伊红染色、免疫组织化学染色及脉络膜血管铺片等;其中以激光诱导的CNV动物模型应用最为广泛,选择合适的造模参数尤为关键。现将各建模方法及成模评价方法综述如下。

Choroidal neovascularization(CNV)is the ultimate pathological manifestation of a variety of fundus diseases.The establishment of CNV animal models with high moldability,good reproducibility,and low cost is of great significance for further research on the pathogenesis,patho‐logical changes,and clinical treatment of CNV.At present,CNV models are genrally induced by laser,subretinal injection of growth factor,and gene.Evaluation methods include fundus fluores‐cein angiograph(FFA)and indocyanine green angiography(ICGA),hematoxylin-eosin staining(HE),immunohistochemistry,and choroidal vascular plating.Laser-induced CNV model is the most widely used,and especially critical to the selection of appropriate modelling parameters.The modeling methods and modeling evaluation methods are summarized as follows.