基于网络药理学分析桑叶增强鸡抗氧化功能的作用机制

Mechanism of Mori Folium in Improving Antioxidative Function of Chicken Based on Network Pharmacology

利用中药系统药理学分析平台(TCMSP)和herb数据库检索收集桑叶的化学成分和靶点,运用Cytoscape3.7.2软件、NCBI数据库、STRING数据库构建并分析桑叶化合物-靶点网络和蛋白质相互作用(PPI)网络,并对靶点进行GO富集和KEGG通路富集分析,藉以分析桑叶增强鸡抗氧化功能的作用机理。结果表明,共筛选到槲皮素、芦丁、山柰酚、β-谷甾醇、β-胡萝卜素和花生四烯酸等30个有效活性成分,这些成分作用于221个靶点。其中IL-6、VEGFA、EGF、INS、CAT、CASP3、CCND1、PTGS2、IL-1B和MMP9等为核心靶点。GO功能富集和KEGG通路分析显示靶点的作用涉及分子功能、生物过程和细胞组成3个方面,并参与到NOD样受体信号通路、过氧化物酶体增殖物激活受体信号通路、Toll样受体信号通路、细胞凋亡等多条代谢通路。本研究表明,理论上桑叶基于IL-6、VEGFA、EGF、INS、CAT、CASP3、CCND1、PTGS2、IL-1B和MMP9等靶点可以调控鸡的抗氧化作用,为进一步的试验验证及桑叶作为抗氧化功能饲料添加剂的开发提供了启示。

This study aims to analyze the mechanism of Mori Folium enhancing antioxidative function of chicken.The chemical components and targets of Mori Folium were collected by the traditional Chinese medicine database and analysis platform(TCMSP)and herb database.The compound-target network and the protein-protein interaction(PPI)network of Mori Folium were constructed and analyzed by Cytoscape3.7.2 software,NCBI database and STRING database.In addition,GO enrichment and KEGG pathway of these targets in PPI network were analyzed.The results showed that a total of 30 active compounds were screened,including quercetin,rutin,kaempferol,β-sitosterol,β-carotene and arachidonic acid,which acted on 221 targets.Among them,IL-6,VEGFA,EGF,INS,CAT,CASP3,CCND1,PTGS2,IL-1B and MMP9 were the core targets.GO enrichment and KEGG pathway analysis showed that the role of the targets involved in molecular function,biological process and cell composition,and involved in a number of metabolic pathways such as NOD-like receptor signaling pathway,PPAR signaling pathway,Toll-like receptor signaling pathway and apoptosis.This study theoretically shows that Mori Folium can regulate the antioxidation of chicken based on targets including IL-6,VEGFA,EGF,INS,CAT,CASP3,CCND1,PTGS2,IL-1B and MMP9,which provides enlightenment for further experimental verification and the development of Mori Folium as antioxidation feed additives.