毛细支气管炎患儿单核细胞TLR4、淋巴细胞CXCR3及血清Mig的表达及临床意义

Expression and significance of monocyte TLR4,lymphocyte CXCR3,and serum Mig in children with bronchiolitis

目的探讨毛细支气管炎患儿单核细胞Toll样受体-4(TLR4)、淋巴细胞趋化因子受体3(CXCR3)及血清干扰素-γ诱导的单核因子(Mig)的表达水平及其临床意义。方法选取2019年1月至2020年5月在宝鸡市妇幼保健院治疗的110例毛细支气管炎患儿作为观察组,其中轻中度患儿65例,重度患儿45例,同时选取宝鸡市妇幼保健院体检的健康儿童50例作为对照组,采用流式细胞仪检测单核细胞TLR4、淋巴细胞CXCR3的表达水平,酶联免疫吸附法检测血清Mig水平。比较各组间LR4、CXCR3和血清Mig水平水平,同时采用Pearson法分析上述各指标间的相关性。结果观察组患儿的TLR4、CXCR3和Mig表达水平分别为(24.40±4.11)ng/mL、(15.50±3.22)ng/mL和(108.28±31.18)pg/mL,明显高于对照组的(1.20±0.32)ng/mL、(12.01±1.82)ng/mL和(65.50±19.22)pg/mL,差异均有统计学意义(P<0.05);观察组中重度组患儿的TLR4、CXCR3和Mig表达水平分别为(26.49±6.01)ng/mL、(17.07±5.03)ng/mL和(121.84±32.20)pg/mL,明显高于轻中度组的(23.02±5.58)ng/mL、(14.41±3.10)ng/mL和(98.89±29.83)pg/mL,差异均有统计学意义(P<0.05);经Pearson法分析结果显示,TLR4与Mig呈正相关(r=0.680,P<0.05),CXCR3与Mig呈正相关(r=0.395,P<0.05),TLR4与CXCR3呈正相关(r=0.396,P<0.05)。结论毛细支气管炎患儿单核细胞TLR4、淋巴细胞CXCR3及血清Mig表达明显升高,且均与患儿病情程度有一定关系,三者间表达呈正相关关系。

Objective To investigate the expression and significance of monocyte Toll like receptor-4(TLR4),lymphocyte chemokine receptor 3(CXCR3),and serum mononuclear factor induced by serum interferon-γ(Mig)in children with bronchiolitis.Methods A total of 110 children with bronchiolitis treated in Baoji Maternal and Child Health Hospital from January 2019 to May 2020 were selected as the observation group,including 65 children with mild/moderate degree bronchiolitis(mild/moderate group)and 45 children with severe degree bronchiolitis(severe group).Fifty healthy children in the Hospital were selected as the control group.The expression levels of TLR4 in monocytes and CXCR3 in lymphocytes were detected by flow cytometry,and the serum Mig level was detected by enzyme-linked immunosorbent assay.The differences of LR4,CXCR3,and serum MIG levels between the two groups were analyzed.Pearson method was used to analyze the correlation between indexes.Results The expression of TLR4,CXCR3,and Mig in the observation group were(24.40±4.11)ng/mL,(15.50±3.22)ng/mL,and(108.28±31.18)pg/mL,which were significantly higher than(1.20±0.32)ng/mL,(12.01±1.82)ng/mL,and(65.50±19.22)pg/mL in the control group(P<0.05).The expression of TLR4,CXCR3,and Mig were(26.49±6.01)ng/mL,(17.07±5.03)ng/mL,and(121.84±32.20)pg/mL in severe group,which were significantly higher than(23.02±5.58)ng/mL,(14.41±3.10)ng/mL,and(98.89±29.83)pg/mL in mild/moderate group(P<0.05).According to the analysis results of Pearson correlation analysis,TLR4 was positively correlated with Mig(r=0.680,P<0.05),CXCR3 was positively correlated with Mig(r=0.395,P<0.05),and TLR4 was positively correlated with CXCR3(r=0.396,P<0.05).Conclusion The expression of TLR4 in monocytes,CXCR3 in lymphocytes,and Mig in serum are significantly increased in children with bronchiolitis,which are positively correlated with the degree of disease.There is a positive correlation among the expression of the three indexes.