摘要
过去,天然过氧化物酶已被成功应用于食品中过氧化氢、葡萄糖、胆固醇等物质的检测。然而,其稳定性差、纯化成本高的缺点限制了其大规模应用。如今,大量过氧化物模拟酶被开发,金属卟啉复合物是其中重要的一种,具有催化活性高、稳定强、成本低等优点。甲烷氧化菌素(methanobactin, Mb)是甲烷氧化菌在限铜条件下分泌的一种铜结合小肽,能够特异性结合铜离子。据报道,甲烷氧化菌素-铜(methanobactin-Cu,Mb-Cu)具有过氧化物酶活性。本文主要介绍金属卟啉过氧化物模拟酶和Mb-Cu过氧化物模拟酶在食品中葡萄糖、胆固醇、多巴胺等生物小分子,以及CaO;、马拉硫磷、铜离子等有害物质的检测中的应用,并重点阐述其中的反应机制,以期为研究人员完善模拟酶设计与合成提供参考。
In the past, natural peroxidase has been successfully used to detect hydrogen peroxide, glucose,cholesterol and other substances in food. However, its large-scale application has been limited due to poor stability and high purification cost. At present, peroxidase mimics have been widely developed, and metalloporphyrin complex is one of the most important ones, which has the advantages of high catalytic activity, strong stability and low cost.Methanobactin(Mb) is a copper-binding small peptide, excreted by methanotrophic bacteria under copper-restricted conditions, which can specifically bind copper ions. It is reported that methanobactin-Cu(Mb-Cu) has peroxidase activity. This paper mainly introduced the application of metalloporphyrin peroxidase mimic enzymes and Mb-Cu peroxidase mimic enzymes in the detection of small biological molecules such as glucose, cholesterol, dopamine and harmful substances such as calcium malathion, phosphorus and copper ions in food, and emphasized their reaction mechanism, so as to provide some help for researchers to improve the design and synthesis of peroxidase mimic enzymes.
作者
方祁利
路雪纯
李慧敏
辛嘉英
夏春谷
FANG Qi-Li;LU Xue-Chun;LI Hui-Min;XIN Jia-Ying;XIA Chun-Gu(Key Laboratory for Food Science and Engineering,Harbin University of Commerce,Harbin 150028,China;State Key Laboratory for Oxo Synthesis&Selective Oxidation,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China)
出处
《食品安全质量检测学报》
CAS
北大核心
2022年第8期2497-2504,共8页
Journal of Food Safety and Quality
基金
黑龙江省自然科学基金项目(LH2020C063)
中央支持地方高校改革发展资金人才培养支持计划项目(高水平人才)(304017)。