基于GO_(x)/NiO@Au-Pt-PGA/GCE酶传感器检测葡萄糖的研究

Detection of glucose based on GO_(x)/NiO@Au-Pt-PGA/GCE enzyme sensor

目的 构建GO;/NiO@Au-Pt-PGA/GCE传感器对果蔬中的葡萄糖快速定量的分析方法。方法 在氧化镍纳米材料(nickel oxide nanomaterials,NiO)的基础上复合金-铂纳米复合材料(gold-platinum nanocomposites,Au-Pt)合成新型NiO@Au-Pt纳米复合材料,以玻碳电极(glassy carbon electrode,GCE)为工作电极,聚谷氨酸[poly-(γ-glutamic acid),PGA]为交联剂交联葡萄糖氧化酶(glucose oxidase,GO;)和NiO@Au-Pt,制备GO;/NiO@Au-Pt-PGA/GCE传感器。通过对交联剂浓度、静置时间的优化确定检测体系最佳工作条件。结果 NiO@Au-Pt具有良好的导电性和催化作用,PGA具有良好的生物相容性和协同作用,GOx/NiO@Au-Pt-PGA/GCE能有效提高传感器的灵敏度,增强电流响应。GOx/NiO@Au-Pt-PGA/GCE的最佳工作条件为1.0%PGA作为制备传感器的交联剂,静置时间20 s。在最佳条件下,葡萄糖浓度与其峰电流在0.01~9.00 mmol/L内呈线性关系,Y=4.1858X+0.169,r^(2)=0.9946,检出限(S/N=3)为2.43μmol/L。GOx/NiO@Au-Pt-PGA/GCE对梨汁中葡萄糖检测结果与国家标准规定的高效液相色谱法一致,且GO;/NiO@Au-Pt-PGA/GCE稳定性较好,4℃保存15 d后其峰电流仅降低了4.89%,对常见的共存干扰物有较好的抗干扰能力。结论 GO;/NiO@Au-Pt-PGA/GCE简单便捷、稳定性好,可用于果蔬中葡萄糖的快速分析。

Objective To establish a GO;/NiO@Au-Pt-PGA/GCE sensor for rapid quantitative analysis of glucose in fruits and vegetables.Methods NiO@Au-Pt nanocomposites were synthesized based on nickel oxide nanomaterials (NiO) by compositing gold-platinum nanocomposites (Au-Pt),GO;/NiO@Au-Pt-PGA/GCE sensor was manufactured using glass carbon electrode (GCE) as working electrode,poly-(γ-glutamic acid)(PGA) as crosslinking agent for crosslinking glucose oxidase (GO;) and NiO@Au-P.The optimum working conditions of the detection system were determined by optimizing the concentration of crosslinking agent and standing time.Results NiO@Au-Pt had good conductivity and catalysis,PGA had good biocompatibility and synergy,GO;/NiO@AuPt-PGA/GCE could effectively improve the sensitivity of the sensor and enhance the current response.The optimum working condition of GO;/NiO@Au-Pt-PGA/GCE was that 1.0%PGA as the crosslinker for preparing the sensor and the standing time was 20 s.Under the optimum conditions,there was a linear relationship between glucose concentration and its peak current in the range of 0.01-9.00 mmol/L,Y=4.1858X+0.169,r~2=0.9946 and the limit of detection (S/N=3) was 2.43μmol/L.The detection results of glucose in pear juice using GO;/NiO@Au-Pt-PGA/GCE was consistent with the result of high performance liquid chromatography stipulated by the national standard,and the stability of GO;/NiO@Au-Pt-PGA/GCE was good,the peak current of GO;/NiO@Au-Pt-PGA/GCE decreased only4.89%after being stored at 4℃for 15 days,and GO;/NiO@Au-Pt-PGA/GCE had good anti-interference ability to common coexistence disturbance.Conclusion GO;/NiO@Au-Pt-PGA/GCE is simple,convenient and stable,and can be used for rapid analysis of glucose in fruits and vegetables.